| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
HA, Nlha (8)
|
NTPSC |
|
48.09 |
- |
| 2 |
Shreve, Peyton N (8)
|
NTDM |
53.71 |
53.95 |
0.24 |
| 3 |
Wright, Ava L (8)
|
NTMTRO |
56.99 |
54.82 |
-2.17 |
| 4 |
Springborn, Molly L (8)
|
NTDM |
55.92 |
56.49 |
0.57 |
| 5 |
Wilson, Elyse H (8)
|
NTJMSS |
59.23 |
57.44 |
-1.79 |
| 6 |
Sears, Ainslee K (7)
|
NTCOPS |
1:02.45 |
58.66 |
-3.79 |
| 7 |
Perez, Bianca D (7)
|
NTSTAR |
1:03.58 |
59.01 |
-4.57 |
| 8 |
Fitzgerald, Taryn S (8)
|
NTCOPS |
1:03.21 |
59.52 |
-3.69 |
| 9 |
Flaming, Gianna E (7)
|
NTMTRO |
1:29.86 |
1:00.27 |
-29.59 |
| 10 |
Parmentier, Vivienne L (7)
|
NTDM |
|
1:01.59 |
- |
| 11 |
Baumgart, Beckley L (8)
|
NTCOPS |
59.65 |
1:01.92 |
2.27 |
| 12 |
Holladay, Hallie A (6)
|
NTATAC |
|
1:04.22 |
- |
| 13 |
Persefield, Abigail E (8)
|
NTATAC |
|
1:06.00 |
- |
| 14 |
Barker, Katherine M (8)
|
NTUN |
1:06.39 |
1:09.29 |
2.90 |
| 15 |
Shaw, Vivien A (8)
|
NTLTSD |
|
1:09.59 |
- |
|
Perez, Brianna C (8)
|
NTSTAR |
1:11.06 |
DQ |
- |
|
Cho, Suin (7)
|
NTPSC |
|
DQ |
- |
|
Kaden, Kinsler C (7)
|
NTATAC |
|
DQ |
- |
|
Howza, Elle R (8)
|
NTMTRO |
|
DQ |
- |
|
Salmassian, Catherine E (6)
|
NTDM |
1:05.37 |
DQ |
- |
|
Pacha, Hannah N (7)
|
NTDM |
|
DQ |
- |
|
Vijey, Hira (8)
|
NTLTSD |
|
NS |
- |
|
Peck, Zoe L (8)
|
NTESP |
58.32 |
NS |
- |