| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kass, Morgan L (12)
|
PVMACH |
29.03 |
29.88 |
0.85 |
| 2 |
Hejmo, Alexandros G (12)
|
PVMACH |
32.06 |
31.12 |
-0.94 |
| 3 |
Ward, Parker A (11)
|
MDASC |
32.41 |
32.02 |
-0.39 |
| 4 |
Mastroianni, Stella (11)
|
PVMACH |
35.67 |
33.87 |
-1.80 |
| 5 |
Armstrong, Katelyn A (11)
|
PVMACH |
37.44 |
36.90 |
-0.54 |
| 6 |
Abney, Luke G (12)
|
PVMACH |
40.89 |
38.53 |
-2.36 |
| 7 |
Branham, Conor J (12)
|
PVMACH |
42.94 |
40.91 |
-2.03 |
| 8 |
Abney, Jake G (12)
|
PVMACH |
44.43 |
41.37 |
-3.06 |
| 9 |
Sarathy, Anoushka (10)
|
PVMACH |
40.95 |
41.47 |
0.52 |
| 10 |
McDonough, Sasha E (11)
|
PVMACH |
46.12 |
42.86 |
-3.26 |
| 11 |
Hersey, Lulu M (9)
|
MDASC |
44.06 |
43.24 |
-0.82 |
| 12 |
Bertin, Benjamin D (10)
|
PVMACH |
48.57 |
45.48 |
-3.09 |
| 13 |
Dalton, Umi J (10)
|
PVMACH |
44.76 |
45.55 |
0.79 |
| 14 |
Roberts Nijim, Sami J (9)
|
PVMACH |
45.39 |
47.40 |
2.01 |
| 15 |
Dober, Leah S (11)
|
PVMACH |
52.91 |
48.41 |
-4.50 |
| 16 |
Ervin, Allison E (9)
|
PVMACH |
1:06.80 |
49.81 |
-16.99 |
| 17 |
Branham, Liam J (10)
|
PVMACH |
57.51 |
55.36 |
-2.15 |