| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
LATINI, SOPHIA (11)
|
MABLUE |
29.90 |
28.95 |
-0.95 |
| 2 |
GIBSON, MADISON A (11)
|
MATRI |
29.70 |
29.01 |
-0.69 |
| 3 |
WATKINS, AZLYNN K (11)
|
MAPSC |
29.21 |
29.06 |
-0.15 |
| 4 |
TEWKSBURY, MADISON O (11)
|
MAPAAC |
30.32 |
29.37 |
-0.95 |
| 5 |
TASSINARI, MARIANNE E (11)
|
MAPFY |
29.31 |
29.67 |
0.36 |
| 6 |
PALOCHIK, AVA C (11)
|
MABLUE |
29.29 |
30.51 |
1.22 |
| 7 |
REINHARD, HAILEY L (11)
|
MAEMAC |
29.91 |
30.94 |
1.03 |
| 8 |
DUDKINA, ELENA V (11)
|
MACBST |
30.83 |
31.09 |
0.26 |
| 9 |
LYNCH, EMILY G (11)
|
MABRY |
30.78 |
31.36 |
0.58 |
| 10 |
BENSON, MEGAN R (11)
|
MABRY |
31.33 |
32.76 |
1.43 |
| 11 |
O'BRIEN, CECILIA M (11)
|
MABLUE |
35.11 |
33.73 |
-1.38 |
| 12 |
KALIHER, CARLITA (11)
|
MABRY |
34.50 |
33.89 |
-0.61 |
| 12 |
HERMAN, ALYVIA L (11)
|
MAPAAC |
34.69 |
33.89 |
-0.80 |
| 14 |
WIRAGH, KENDALL J (11)
|
MAEMAC |
37.44 |
36.98 |
-0.46 |
| 15 |
TALIJAN, JULIANNE L (11)
|
MABLUE |
36.07 |
37.59 |
1.52 |
| 16 |
LATINI, ISABELLA R (11)
|
MABLUE |
41.48 |
39.58 |
-1.90 |