| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gibson, Grace A (10)
|
MANRG |
|
45.20 |
- |
| 1 |
Kilyk, Miranda (10)
|
MAMUHL |
45.99 |
47.23 |
1.24 |
| 2 |
Fleischaker, Mazie R (9)
|
MAPAAC |
48.23 |
48.00 |
-0.23 |
| 3 |
Fegely, Norah (9)
|
MAMUHL |
48.04 |
49.03 |
0.99 |
| 4 |
Wicker, Anna G (10)
|
MANRG |
50.88 |
49.55 |
-1.33 |
| 5 |
Wegner, Abigail (10)
|
MATRA |
|
51.11 |
- |
| 6 |
Tewksbury, Victoria E (9)
|
MAPAAC |
56.18 |
53.01 |
-3.17 |
| 7 |
Khais, Anna N (10)
|
MAPAAC |
56.21 |
53.82 |
-2.39 |
| 8 |
Smith, Shannon M (9)
|
MANRG |
|
54.18 |
- |
| 9 |
Barbehenn, Hannah G (9)
|
MAEMAC |
57.33 |
55.94 |
-1.39 |
| 10 |
McAllister, Samira E (10)
|
MANRG |
|
56.14 |
- |
| 11 |
Razavi, Lily G (10)
|
MAPAAC |
52.68 |
56.32 |
3.64 |
| 12 |
Rogers, Emma L (10)
|
MAKIDS |
55.80 |
56.97 |
1.17 |
| 13 |
Rojas, Norah J (9)
|
MAPAAC |
57.36 |
57.56 |
0.20 |
| 14 |
Woods, Isabella J (10)
|
MAPAAC |
1:00.66 |
1:02.69 |
2.03 |
| 15 |
Brion, Katherine E (10)
|
MAPAAC |
1:28.95 |
1:03.46 |
-25.49 |
| 16 |
Soehnlen, Alyssa D (9)
|
MAPAAC |
|
1:03.50 |
- |
| 17 |
Tsarouhis, Sophia E (9)
|
MAPAAC |
1:06.55 |
1:04.70 |
-1.85 |
| 18 |
Tsarouhis, Paulina M (9)
|
MAPAAC |
1:03.00 |
1:04.87 |
1.87 |