| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DiMaio, Mya S (13)
|
AMRGSY |
2:43.53 |
2:38.05 |
-5.48 |
| 2 |
Schomaker, Allison H (13)
|
AMPRA |
2:37.80 |
2:40.19 |
2.39 |
| 3 |
Poe, Abby S (14)
|
AMPEAQ |
2:44.38 |
2:40.52 |
-3.86 |
| 4 |
Klingensmith, Hanna L (14)
|
AMPEAQ |
2:45.47 |
2:40.62 |
-4.85 |
| 5 |
Melocchi, Grace R (14)
|
AMPEAQ |
2:42.19 |
2:43.19 |
1.00 |
| 6 |
Ulintz, Anastasia M (14)
|
AMSTEE |
2:42.86 |
2:46.54 |
3.68 |
| 7 |
Laurenti, Gianna M (13)
|
AMMMSA |
2:50.20 |
2:46.79 |
-3.41 |
| 8 |
Bowers, Addison R (14)
|
AMPTSC |
2:47.66 |
2:47.78 |
0.12 |
| 9 |
Linge, Katherine G (13)
|
AMSTEE |
2:57.72 |
2:53.12 |
-4.60 |
| 10 |
Sheets, Elizabeth A (14)
|
AMHD |
3:08.23 |
2:55.12 |
-13.11 |
| 11 |
Daugherty, Maya J (14)
|
AMHD |
2:58.90 |
3:04.59 |
5.69 |
| 12 |
Bruce, Madison S (14)
|
AMHD |
3:10.83 |
3:05.29 |
-5.54 |
| 13 |
Byars, Adeline F (13)
|
AMNAC |
3:06.28 |
3:05.90 |
-0.38 |
| 14 |
Dudkowski, Abigail E (14)
|
AMPRA |
3:10.28 |
3:10.36 |
0.08 |