| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Plank, Anna R (14)
|
AMSRST |
2:22.89 |
2:26.98 |
4.09 |
| 2 |
Khoury, Rita M (14)
|
AMHD |
2:26.61 |
2:29.81 |
3.20 |
| 3 |
Anderson, Ariana A (13)
|
AMPRA |
2:23.07 |
2:33.25 |
10.18 |
| 4 |
Massella, Maggie E (14)
|
AMSRST |
2:34.17 |
2:36.47 |
2.30 |
| 5 |
LaRoche, Francesca L (14)
|
AMWHAT |
2:33.85 |
2:40.60 |
6.75 |
| 6 |
Wienand, Paige L (13)
|
AMMMSA |
2:46.12 |
2:43.10 |
-3.02 |
| 7 |
Lange, Paige M (13)
|
AMNHA |
2:40.56 |
2:43.32 |
2.76 |
| 8 |
Harris, Emma R (13)
|
AMPTAC |
2:48.34 |
2:46.03 |
-2.31 |
| 9 |
Mushinsky, Ava E (14)
|
AMHD |
2:58.76 |
2:53.24 |
-5.52 |
| 10 |
Tucker, Angela M (14)
|
AMCSC |
2:53.44 |
2:53.67 |
0.23 |
| 11 |
Michael, Lauren C (14)
|
AMHD |
3:09.79 |
3:01.49 |
-8.30 |
| 12 |
Strasser, Scarlet Sophia F (13)
|
AMMMSA |
2:50.99 |
3:01.92 |
10.93 |
|
Hutchison, Isabella N (13)
|
AMCSC |
3:15.21 |
DQ |
- |
|
Tew, Annabel P (13)
|
AMJCCS |
3:03.60 |
NS |
- |
|
Albertson, Jessica A (13)
|
AMPRA |
3:01.18 |
NS |
- |
|
Reed, Ella A (14)
|
AMJCCS |
2:55.08 |
NS |
- |
|
Spiess, Emma E (13)
|
AMHD |
2:37.13 |
NS |
- |