| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Plank, Cora J (12)
|
AMSRST |
2:27.74 |
2:28.73 |
0.99 |
| 2 |
FaJohn, Raely D (12)
|
AMSRST |
2:30.27 |
2:32.41 |
2.14 |
| 3 |
Schomaker, Anna J (12)
|
AMPRA |
2:29.89 |
2:36.18 |
6.29 |
| 4 |
Shafer, Riley A (11)
|
AMPRA |
2:48.12 |
2:38.22 |
-9.90 |
| 5 |
Schomaker, Allison H (11)
|
AMPRA |
3:20.58 |
2:40.66 |
-39.92 |
| 6 |
Pietropaolo, Sophia G (12)
|
AMPRA |
2:45.50 |
2:40.71 |
-4.79 |
| 7 |
Sheets, Elizabeth A (12)
|
AMHD |
2:42.26 |
2:47.45 |
5.19 |
| 8 |
Gregorowicz, Abigail S (12)
|
AMPRA |
3:10.67 |
2:51.68 |
-18.99 |
| 9 |
Rupert, Brooke T (11)
|
AMPRA |
3:00.67 |
2:52.15 |
-8.52 |
| 10 |
Laurenti, Gianna M (11)
|
AMMMSA |
2:57.99 |
2:52.95 |
-5.04 |
| 11 |
Bruce, Madison S (11)
|
AMHD |
2:54.24 |
2:53.60 |
-0.64 |
| 12 |
Urling, Alyssa C (11)
|
AMPRA |
3:02.13 |
2:59.88 |
-2.25 |
| 13 |
Herron, Aubrey R (11)
|
AMSRST |
2:56.23 |
3:01.57 |
5.34 |
| 14 |
Roth, Ella (12)
|
AMHD |
3:12.96 |
3:03.84 |
-9.12 |
|
Siegel, Thea E (11)
|
AMHD |
2:36.29 |
NS |
- |