| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Broeker, Max E (10)
|
NIWWSC |
47.31 |
39.27 |
-8.04 |
| 1 |
Labiak, Sean A (10)
|
NIFALC |
42.19 |
41.40 |
-0.79 |
| 1 |
Nienhaus, Matthew (9)
|
NISHAQ |
48.25 |
45.98 |
-2.27 |
| 2 |
Reygers, Camden M (10)
|
NIWWSC |
46.21 |
42.04 |
-4.17 |
| 2 |
Sullivan, Nolan M (10)
|
NIHAMB |
48.87 |
46.28 |
-2.59 |
| 3 |
Donlon, Joseph R (10)
|
NIWWSC |
44.99 |
44.12 |
-0.87 |
| 3 |
Lemieux, Keaton D (10)
|
NIFALC |
48.95 |
46.69 |
-2.26 |
| 4 |
Ruffolo, Trenton J (9)
|
NIWWSC |
45.32 |
44.65 |
-0.67 |
| 4 |
Hagos, Eyobel (10)
|
NITTSC |
43.09 |
46.96 |
3.87 |
| 5 |
Liebenow, Miles R (10)
|
NIHAMB |
42.38 |
44.94 |
2.56 |
| 5 |
Charlier, Andre' D (9)
|
NITTSC |
43.64 |
48.62 |
4.98 |
| 6 |
Askar, Tommy J (10)
|
NIHHSC |
51.55 |
49.10 |
-2.45 |
| 7 |
Zuckerberg, Eben M (10)
|
NITTSC |
52.16 |
49.94 |
-2.22 |
| 8 |
Moceri, Michael J (9)
|
NIFALC |
1:06.88 |
50.39 |
-16.49 |
| 9 |
Olsen, Owen T (10)
|
NIAMTS |
54.25 |
52.65 |
-1.60 |
| 10 |
Devans, Aidan R (10)
|
NIRAYS |
1:01.34 |
52.77 |
-8.57 |
| 11 |
Gaia, Thomas M (10)
|
NISHAQ |
|
53.72 |
- |
| 12 |
Bibler, William S (9)
|
NICLSC |
|
55.20 |
- |
| 13 |
Bauman, Jackson T (9)
|
NISHAQ |
|
59.26 |
- |
| 14 |
Colosi-Marcantonio, Hayden C (9)
|
NIHAMB |
|
1:00.62 |
- |
| 15 |
Wisniewski, Jordan R (10)
|
NIRAYS |
|
1:12.66 |
- |
|
Nieman, Zach (10)
|
NISHAQ |
51.85 |
DQ |
- |
|
Densmore, Connor M (10)
|
NICLSC |
43.88 |
DQ |
- |