| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ries, Alexandria R (10)
|
PVPM |
33.49 |
34.03 |
0.54 |
| 2 |
Wilhite, Charlotte L (10)
|
PVMACH |
35.12 |
34.40 |
-0.72 |
| 3 |
Young, Aida H (10)
|
PVCSC |
33.70 |
34.95 |
1.25 |
| 4 |
Charters, Shannon A (10)
|
PVMACH |
36.90 |
35.37 |
-1.53 |
| 5 |
Callahan, Grace H (10)
|
PVMACH |
34.85 |
35.68 |
0.83 |
| 6 |
Klotz, Anna C (10)
|
PVBWST |
|
36.08 |
- |
| 7 |
Estes, Kaitlyn M (10)
|
PVSNOW |
36.31 |
36.13 |
-0.18 |
| 8 |
Sims, Marisol W (10)
|
PVSNOW |
38.40 |
37.32 |
-1.08 |
| 9 |
Schloth, Katherine I (10)
|
PVPM |
37.09 |
37.35 |
0.26 |
| 10 |
Davenhall, Kiersten E (10)
|
PVSNOW |
39.51 |
38.26 |
-1.25 |
| 11 |
He, Emma C (9)
|
PVPM |
|
39.84 |
- |
| 12 |
Gartzke, Monika T (10)
|
PVCSC |
|
39.97 |
- |
| 13 |
Hayward, Audrey I (8)
|
PVBWST |
|
40.15 |
- |
| 14 |
Crapco-Wang, Caitlyn L (10)
|
PVMACH |
51.22 |
41.69 |
-9.53 |
| 15 |
Hanlin, Carly A (10)
|
PVBWST |
43.53 |
41.78 |
-1.75 |
| 16 |
Lim, Charlotte M (9)
|
PVMAKO |
40.67 |
41.84 |
1.17 |
| 17 |
Levin, Ava E (10)
|
PVBWST |
41.78 |
43.32 |
1.54 |
| 18 |
Du, Madelyn (10)
|
PVMACH |
|
43.57 |
- |
| 19 |
Fassacesia, Giada L (10)
|
PVBWST |
43.90 |
43.86 |
-0.04 |
| 20 |
Borley, Charlotte R (9)
|
PVBWST |
45.63 |
44.59 |
-1.04 |
| 21 |
McAllister, Maia C (9)
|
PVPM |
49.11 |
47.16 |
-1.95 |
| 22 |
Pollock, Claire M (9)
|
PVBWST |
|
47.25 |
- |
| 23 |
McCurry, Eva M (10)
|
PVSNOW |
46.80 |
48.07 |
1.27 |
| 24 |
Miller, Avery R (8)
|
PVMAKO |
53.26 |
50.03 |
-3.23 |
| 25 |
Bendza, Corinne J (6)
|
PVMACH |
|
53.75 |
- |