| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thornton, Kira N (13)
|
PVMACH |
2:37.85 |
2:42.18 |
4.33 |
| 2 |
Jingan, Jinglin (14)
|
PVFISH |
|
2:43.65 |
- |
| 3 |
Kang, Gianna H (14)
|
PVNCAP |
2:40.72 |
2:45.48 |
4.76 |
| 4 |
Rieger, Annika L (13)
|
PVMACH |
|
2:48.37 |
- |
| 5 |
Guthrie, Sydney E (14)
|
PVFISH |
2:48.33 |
2:51.30 |
2.97 |
| 6 |
Knaggs, Claire E (14)
|
PVMACH |
2:46.61 |
2:51.84 |
5.23 |
| 7 |
Diccox, Sophie A (13)
|
PVMACH |
2:51.93 |
2:57.46 |
5.53 |
| 8 |
Wentland, Annika M (13)
|
PVNCAP |
3:07.44 |
2:57.69 |
-9.75 |
| 9 |
Landeryou, Clara E (13)
|
PVNCAP |
|
2:58.60 |
- |
| 10 |
Clodfelter, Charlotte R (13)
|
PVLIFE |
|
3:00.26 |
- |
| 11 |
Watson, Marina M (13)
|
PVFISH |
|
3:00.70 |
- |
| 12 |
Crawford, Katherine V (13)
|
PVFISH |
2:57.64 |
3:01.12 |
3.48 |
| 13 |
Betlack, Laura M (14)
|
PVLIFE |
3:07.91 |
3:06.46 |
-1.45 |
| 14 |
White, Grace E (14)
|
PVFBST |
3:04.03 |
3:07.65 |
3.62 |
| 15 |
Hans, Lara Y (13)
|
PVNCAP |
3:05.59 |
3:10.99 |
5.40 |
| 16 |
Liu, Isabel X (13)
|
PVFISH |
|
3:11.76 |
- |
| 17 |
Tysse, Ella R (13)
|
PVNCAP |
|
3:38.16 |
- |
|
Martell, Caroline A (14)
|
PVMACH |
3:04.25 |
NS |
- |
|
Janczyk, Megan D (13)
|
PVMACH |
2:45.60 |
NS |
- |