| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Charlotte T (12)
|
MRNDAC |
37.93 |
38.20 |
0.27 |
| 2 |
Boyd, Madison E (11)
|
MRNDAC |
46.48 |
45.02 |
-1.46 |
| 3 |
Sanford, Amelia J (12)
|
MRNDAC |
44.03 |
45.39 |
1.36 |
| 4 |
McCluskey, Jayden K (11)
|
MRNDAC |
48.46 |
46.24 |
-2.22 |
| 5 |
Murray, Megan (11)
|
MRAG |
48.85 |
46.44 |
-2.41 |
| 6 |
Spinelli, Bianca C (11)
|
MRNDAC |
47.06 |
46.70 |
-0.36 |
| 7 |
Kasnia-Herman, Kaylani G (11)
|
MRNDAC |
50.41 |
47.74 |
-2.67 |
| 8 |
Ault Nolan, McKenna M (12)
|
MRNDAC |
51.66 |
47.93 |
-3.73 |
| 9 |
Mastandrea, Amanda N (11)
|
MRAG |
50.63 |
49.69 |
-0.94 |
| 10 |
Reyes, Marina L (11)
|
MRNDAC |
55.55 |
55.70 |
0.15 |
| 11 |
Seldin, Emily (11)
|
MRNDAC |
53.37 |
56.07 |
2.70 |
| 12 |
Hewitt, Allison C (11)
|
MRNDAC |
|
56.17 |
- |
|
Kuenster, Maya S (12)
|
MRNDAC |
|
NS |
- |