| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Humphrey, Chloe E (15)
|
NTUN |
2:26.96 |
2:27.48 |
0.52 |
| 2 |
Houdashell, Maya B (13)
|
NTMAC |
2:32.33 |
2:27.56 |
-4.77 |
| 3 |
Reed, Paige I (13)
|
NTESP |
2:38.48 |
2:35.99 |
-2.49 |
| 4 |
Kruzel, Peighton J (13)
|
NTMARS |
2:36.79 |
2:37.55 |
0.76 |
| 5 |
Fox, Evelyn P (14)
|
NTESP |
2:37.76 |
2:37.94 |
0.18 |
| 6 |
Mills, Ashlyn M (13)
|
NTMAC |
2:41.75 |
2:38.67 |
-3.08 |
| 7 |
Griffith, Rachel R (13)
|
NTUN-P |
2:42.19 |
2:42.17 |
-0.02 |
| 8 |
Kruse, Kylie L (13)
|
NTMAC |
2:46.77 |
2:42.88 |
-3.89 |
| 9 |
Ferguson, Kaitlyn M (13)
|
NTMAC |
2:45.01 |
2:43.47 |
-1.54 |
| 10 |
Hall, Zoey C (14)
|
NTFNW |
2:46.10 |
2:44.54 |
-1.56 |
| 11 |
Nsabimana, Gabrielle N (13)
|
NTMAC |
3:01.99 |
2:45.03 |
-16.96 |
| 12 |
Menezes, Anisha (14)
|
NTMARS |
2:45.78 |
2:45.99 |
0.21 |
| 13 |
Campbell, Eliana R (14)
|
NTESP |
2:47.21 |
2:46.75 |
-0.46 |
| 14 |
Brough, Regina C (13)
|
NTMAC |
2:52.86 |
2:47.50 |
-5.36 |
| 15 |
Drori, Sarah (13)
|
NTMARS |
2:50.30 |
2:51.37 |
1.07 |
| 16 |
Nguyen, Leni Q (14)
|
NTMAC |
2:51.37 |
2:52.54 |
1.17 |
| 17 |
Hunter, Margaret Q (14)
|
NTMAC |
2:57.23 |
2:52.63 |
-4.60 |
| 18 |
Constantine, Katherine E (13)
|
NTMARS |
2:56.25 |
2:53.08 |
-3.17 |
| 19 |
Dietrich, Hannah K (13)
|
NTFAST |
2:51.71 |
2:53.77 |
2.06 |
| 20 |
Luna, Alexandra L (14)
|
NTMAC |
2:50.93 |
2:54.33 |
3.40 |
| 21 |
Calavan, Emily J (15)
|
NTMTRO |
3:05.03 |
2:54.93 |
-10.10 |
| 22 |
Cating, Anna R (13)
|
NTJMSS |
2:55.19 |
2:57.75 |
2.56 |
| 23 |
Nguyen, Lana T (14)
|
NTMAC |
2:52.95 |
2:58.39 |
5.44 |
| 24 |
Vu, Sarah M (15)
|
NTMARS |
3:24.19 |
3:10.19 |
-14.00 |
| 25 |
Hebert, Andrea N (16)
|
NTO2 |
3:23.13 |
3:22.89 |
-0.24 |
| 26 |
Das, Anuhya A (13)
|
NTO2 |
|
3:36.48 |
- |
|
Eubank, Halle K (14)
|
NTMAC |
|
DQ |
- |
|
Smith, Helena N (13)
|
NTMARS |
3:39.48 |
NS |
- |
|
Swinarsky, Caroline G (13)
|
NTMARS |
|
NS |
- |