| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Horner, Mckenzie G (7)
|
PVMACH |
47.28 |
39.83 |
-7.45 |
| 2 |
Zhao, Ellie (7)
|
PVFISH |
51.12 |
45.48 |
-5.64 |
| 3 |
Celentano, Adelaide F (7)
|
PVAAC |
|
45.75 |
- |
| 4 |
Wiesbauer, Amelie K (7)
|
PVNCAP |
|
46.36 |
- |
| 5 |
McKechnie, Marilyn F (7)
|
PVMACH |
|
46.92 |
- |
| 6 |
Batten, Avery E (7)
|
PVMACH |
|
47.66 |
- |
| 7 |
Blank, Brinkley C (7)
|
PVNCAP |
|
49.64 |
- |
| 8 |
Russell, Quinn K (7)
|
PVASA |
49.08 |
49.83 |
0.75 |
| 9 |
Mamillapalli, Ruthvika S (7)
|
PVMACH |
58.59 |
49.98 |
-8.61 |
| 10 |
Allen, Kailyn M (7)
|
PVHACC |
|
50.93 |
- |
| 11 |
Patrick, Ellen M (7)
|
PVASA |
58.55 |
51.15 |
-7.40 |
| 12 |
Bruley, Hailey N (7)
|
PVBWST |
51.26 |
51.55 |
0.29 |
| 13 |
Calway, Harriet M (7)
|
PVMACH |
|
51.63 |
- |
| 14 |
Palacio, Isabella (7)
|
PVNCAP |
|
51.65 |
- |
| 15 |
Haddad, Joely A (7)
|
PVASA |
57.02 |
52.70 |
-4.32 |
| 16 |
Spaisman, Sloan A (7)
|
PVASA |
56.46 |
53.55 |
-2.91 |
| 17 |
Krueger, Clementine L (7)
|
PVFISH |
1:10.23 |
54.57 |
-15.66 |
| 18 |
Bloss, Madelyn G (7)
|
PVAAC |
1:00.16 |
55.19 |
-4.97 |
| 19 |
McCready, Liliana L (7)
|
PVAAC |
|
56.08 |
- |
| 20 |
Naransumber, Zoey T (7)
|
PVAAC |
|
58.84 |
- |
| 21 |
Nolly, Yatra (7)
|
PVAAC |
|
59.04 |
- |
| 22 |
Amodio, Gia J (7)
|
PVBWST |
|
1:01.10 |
- |
| 23 |
Bricker, Alison J (7)
|
PVFISH |
1:12.06 |
1:02.29 |
-9.77 |
| 24 |
Suthard, Valerie (7)
|
PVSTJS |
|
1:02.71 |
- |
| 25 |
Thompson, Mary E (7)
|
PVAAC |
21.13 |
1:03.97 |
42.84 |
| 26 |
Mirzayan, Ariana G (7)
|
PVMACH |
|
1:08.62 |
- |
| 27 |
Battumur, Kennedy A (7)
|
PVAAC |
|
1:09.96 |
- |
| 28 |
Dasenbrook, Sarah C (7)
|
PVAAC |
|
1:15.56 |
- |
| 29 |
Henrique, Ava F (7)
|
PVAAC |
|
1:17.15 |
- |
|
Lundgren, Anna J (7)
|
PVMACH |
|
NS |
- |