| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schere, Fiona D (17)
|
MDNBAC |
27.47 |
27.46 |
-0.01 |
| 2 |
Nwakalor, Melissa O (16)
|
MDNBAC |
28.69 |
28.60 |
-0.09 |
| 3 |
Dopson, Mia B (18)
|
MDNBAC |
27.64 |
28.63 |
0.99 |
| 4 |
Weker, Zoe M (15)
|
MDRAC |
33.16 |
29.03 |
-4.13 |
| 5 |
Alaish, Gabriella G (17)
|
MDNBAC |
27.70 |
29.70 |
2.00 |
| 6 |
Wilson, Kendal G (17)
|
MDRAC |
30.71 |
30.29 |
-0.42 |
| 7 |
Hogue, Sofie M (16)
|
MDRAC |
29.99 |
30.34 |
0.35 |
| 8 |
Rippeon, Genevieve N (15)
|
MDEST |
34.72 |
31.50 |
-3.22 |
| 9 |
Turk, Sydney E (16)
|
MDLBA |
30.43 |
31.94 |
1.51 |
| 10 |
Nettles, Brionn K (17)
|
MDEST |
29.74 |
32.09 |
2.35 |
| 11 |
Weaver, Kyleigh N (18)
|
MDACSS |
31.00 |
32.22 |
1.22 |
| 12 |
Burke, Mackenzie N (15)
|
MDEST |
32.29 |
32.52 |
0.23 |
| 13 |
Marquez, Mary A (15)
|
MDNBAC |
31.51 |
32.62 |
1.11 |
| 14 |
Birdsall, Caroline R (15)
|
MDEST |
32.70 |
32.94 |
0.24 |
| 15 |
Bruning, Riley C (17)
|
MDNBAC |
33.00 |
33.49 |
0.49 |
| 16 |
Hudson, Ava K (17)
|
MDHFY |
34.45 |
34.49 |
0.04 |
| 17 |
Price, Isabella (15)
|
MDHFY |
33.75 |
35.22 |
1.47 |
|
Kurzatkowski, Julia (15)
|
MDNBAC |
30.32 |
NS |
- |
|
Rollins, Heather C (16)
|
MDRAC |
|
NS |
- |
|
Sesker, Ryan O (17)
|
MDASC |
|
NS |
- |