| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Liu, Kevin S (11)
|
MDCAA |
35.34 |
36.54 |
1.20 |
| 2 |
Merke, Matthew J (11)
|
MDCAA |
40.83 |
37.52 |
-3.31 |
| 3 |
Zhong, Nathan Y (11)
|
MDCAA |
43.46 |
39.36 |
-4.10 |
| 4 |
Alexander, Finn W (11)
|
MDRAC |
38.22 |
39.70 |
1.48 |
| 5 |
Hoang, Jackson M (11)
|
MDHAGY |
39.36 |
39.89 |
0.53 |
| 6 |
Davies, Cody J (11)
|
MDUN |
42.94 |
42.08 |
-0.86 |
| 7 |
Cason, Ibraheem B (11)
|
MDBCSC |
41.87 |
42.81 |
0.94 |
| 8 |
Rowland, Logan N (11)
|
MDMAC |
47.39 |
43.11 |
-4.28 |
| 9 |
Hartman, Alexander R (11)
|
MDCAA |
|
44.54 |
- |
| 10 |
Greene, Andrew E (11)
|
MDSPRC |
51.92 |
44.63 |
-7.29 |
| 11 |
Mitchell, Lucas P (11)
|
MDMAC |
46.61 |
44.93 |
-1.68 |
| 12 |
Langnas, Evan C (11)
|
MDCAA |
46.72 |
45.45 |
-1.27 |
| 13 |
Harvey, Jeffrey C (11)
|
MDGBSA |
46.40 |
46.02 |
-0.38 |
| 14 |
Wang, Arthur T (11)
|
MDCAA |
47.99 |
47.06 |
-0.93 |
| 15 |
Musante, Nicholas P (11)
|
MDACA |
50.19 |
47.27 |
-2.92 |
| 16 |
Sotirelis, Demetrios T (11)
|
MDRAC |
50.21 |
47.47 |
-2.74 |
| 17 |
Volinsky, Elan (11)
|
MDJCC |
50.73 |
50.22 |
-0.51 |
| 18 |
Keogh, Timothy A (11)
|
MDCAA |
50.17 |
50.72 |
0.55 |
| 19 |
James, Ayden N (11)
|
MDHSST |
51.08 |
52.10 |
1.02 |
| 20 |
Miller, John H (11)
|
MDHSST |
53.61 |
52.39 |
-1.22 |
|
Walden, Chandler E (11)
|
MDSPRC |
1:24.54 |
DQ |
- |
|
Vahlsing, Joshua J (11)
|
MDRAC |
46.04 |
NS |
- |