| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brooks, Mary G (15)
|
MDCGA |
31.78 |
30.81 |
-0.97 |
| 2 |
Lingelbach, Sarah E (15)
|
MDACA |
32.40 |
31.30 |
-1.10 |
| 3 |
Vu, Amanda P (15)
|
MDASC |
28.81 |
31.73 |
2.92 |
| 3 |
Hatley, Samara K (15)
|
MDASC |
32.01 |
31.73 |
-0.28 |
| 5 |
Melchionni, Madeline H (15)
|
MDMAS |
32.09 |
31.97 |
-0.12 |
| 6 |
Milter, Michelle E (15)
|
UNAT |
31.96 |
32.25 |
0.29 |
| 7 |
Liu, Jenica Y (15)
|
MDRAC |
32.79 |
32.35 |
-0.44 |
| 8 |
Taylor, Emma K (15)
|
MDCGA |
32.67 |
32.51 |
-0.16 |
| 9 |
DeSantis, Lauren I (15)
|
MDCGA |
33.68 |
33.54 |
-0.14 |
| 10 |
Valkenberg, Sophia H (16)
|
MDMSC |
32.74 |
34.06 |
1.32 |
| 11 |
Smith, Aria C (15)
|
MDGBSA |
34.61 |
35.02 |
0.41 |
| 12 |
Duffy, Abbygale R (15)
|
MDGBSA |
34.12 |
35.77 |
1.65 |
| 13 |
Woods, Mary D (15)
|
MDGBSA |
32.67 |
36.21 |
3.54 |
| 14 |
Dick, Ashley E (16)
|
MDMAS |
36.62 |
37.27 |
0.65 |
| 15 |
Muhammad, Ndera S (15)
|
MDBCSC |
45.55 |
44.25 |
-1.30 |
|
Stubblefield, Grace M (15)
|
MDCGA |
32.23 |
NS |
- |
|
Li, Megan H (15)
|
MDCAA |
33.31 |
NS |
- |
|
Trexler, Margaux G (16)
|
MDMSC |
32.48 |
NS |
- |