| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Varelli, Anna C (14)
|
MDMAS |
42.52 |
31.26 |
-11.26 |
| 2 |
Lueders, Grace E (13)
|
MDBVA |
33.52 |
31.40 |
-2.12 |
| 3 |
Aliftiras, Elisabeth G (13)
|
MDFSC |
31.67 |
31.65 |
-0.02 |
| 4 |
Watson, Lauren S (13)
|
MDMAS |
33.88 |
33.57 |
-0.31 |
| 5 |
Oswald, Mary K (13)
|
MDMAS |
33.01 |
34.26 |
1.25 |
| 6 |
Roark, Olivia M (14)
|
MDFAST |
33.16 |
34.31 |
1.15 |
| 7 |
Plummer, Gabriela R (14)
|
MDFAST |
36.42 |
36.75 |
0.33 |
| 8 |
Presley, Madison A (13)
|
MDMAS |
35.29 |
38.00 |
2.71 |
| 9 |
Treeman-Reinke, Ariadne I (13)
|
MDBVA |
40.91 |
39.17 |
-1.74 |
| 10 |
Magnuson, Autumn D (13)
|
MDNWSC |
1:05.02 |
44.95 |
-20.07 |
|
Clark, Destiny (13)
|
MDAESC |
36.36 |
NS |
- |