| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Masse, Chloe G (12)
|
MEMMD |
27.53 |
27.04 |
-0.49 |
| 2 |
Hammer, Layla J (12)
|
MELRSC |
27.53 |
27.10 |
-0.43 |
| 3 |
Maksimova, Aleksandra P (12)
|
MECMA |
27.93 |
28.02 |
0.09 |
| 4 |
Weeks, Avery W (12)
|
MEPBAY |
30.28 |
28.77 |
-1.51 |
| 5 |
Carleton, Charley S (11)
|
MELRSC |
28.24 |
28.84 |
0.60 |
| 6 |
Dew, Elisa E (11)
|
MECMA |
28.60 |
28.95 |
0.35 |
| 7 |
Willey, Jessie E (12)
|
MEWCCU |
29.73 |
29.30 |
-0.43 |
| 8 |
Colaluca, Olivia M (12)
|
MELRSC |
29.81 |
29.76 |
-0.05 |
| 9 |
Layton, Elizabeth F (11)
|
MEPBAY |
30.79 |
29.95 |
-0.84 |
| 10 |
Perry, Vivienne (11)
|
MEPBAY |
31.19 |
30.06 |
-1.13 |
| 11 |
Rodriguez, Yuiza A (12)
|
MELRSC |
30.47 |
31.30 |
0.83 |
| 12 |
Levy, Eliza R (12)
|
MELRSC |
32.43 |
31.89 |
-0.54 |
| 13 |
McLaughlin, McKaela M (12)
|
MEMMD |
31.54 |
32.27 |
0.73 |
| 14 |
Cloutier, Klara T (12)
|
MEYWTS |
33.16 |
33.43 |
0.27 |