| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gould, Charles Q (14)
|
ORLOSC |
56.28 |
57.83 |
1.55 |
| 2 |
Nimase, Ojas S (14)
|
ORBLAC |
59.69 |
59.03 |
-0.66 |
| 3 |
Settle, Charlie M (14)
|
ORBLAC |
1:01.41 |
1:00.67 |
-0.74 |
| 4 |
Yang, Jiyu (14)
|
ORLOSC |
58.77 |
1:01.04 |
2.27 |
| 5 |
Deitchler, Zachary B (13)
|
ORLOSC |
1:06.17 |
1:02.32 |
-3.85 |
| 6 |
Naim, Joshua A (14)
|
PCDACA |
1:01.93 |
1:03.98 |
2.05 |
| 7 |
Bruss, Darian J (14)
|
ORBLAC |
1:04.51 |
1:04.24 |
-0.27 |
| 8 |
Bouali, Yassin I (13)
|
PCDACA |
1:06.30 |
1:04.56 |
-1.74 |
| 9 |
Everitt, Benjamin D (14)
|
PCDACA |
1:05.59 |
1:05.11 |
-0.48 |
| 10 |
Kabacy, Alexander E (14)
|
PNSMAC |
1:08.11 |
1:06.32 |
-1.79 |
| 11 |
Park, Gregory J (13)
|
ORLOSC |
1:08.81 |
1:07.27 |
-1.54 |
| 12 |
Smith, Carson K (13)
|
PCDACA |
1:08.20 |
1:07.80 |
-0.40 |
| 13 |
Ma, Jaden F (13)
|
PNSMAC |
1:10.80 |
1:08.00 |
-2.80 |
| 14 |
Stahlmann, Benjamin J (14)
|
ORBLAC |
1:12.15 |
1:08.16 |
-3.99 |