| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kayfes, Willa R (10)
|
ORTHSC |
37.75 |
37.01 |
-0.74 |
| 2 |
Huettemeyer, Helena R (10)
|
ORLOSC |
39.84 |
39.75 |
-0.09 |
| 2 |
McDowell, Ava R (10)
|
ORLOSC |
40.57 |
39.75 |
-0.82 |
| 4 |
Tran, Amanda (10)
|
PCDACA |
42.55 |
40.64 |
-1.91 |
| 5 |
Patel, Rhianna M (10)
|
ORLOSC |
44.43 |
43.21 |
-1.22 |
| 6 |
Wong, Zoe H (9)
|
PCDACA |
34.16 |
44.21 |
10.05 |
| 7 |
Uchishiba-Hall, Emelia T (9)
|
ORLOSC |
45.48 |
44.30 |
-1.18 |
| 8 |
Fanucchi, Lauren E (8)
|
PCDACA |
45.38 |
45.31 |
-0.07 |
| 9 |
Kokoori, Shankari (10)
|
ORTHSC |
48.20 |
46.51 |
-1.69 |
| 10 |
Levin, Liliana M (9)
|
PCDACA |
45.74 |
48.26 |
2.52 |
| 11 |
Park, Yeha (9)
|
ORTHSC |
49.47 |
48.78 |
-0.69 |
| 12 |
Wen, Julia C (9)
|
ORTHSC |
45.98 |
48.97 |
2.99 |
| 13 |
Lim, Jihoo (9)
|
ORTHSC |
52.56 |
52.94 |
0.38 |
|
Nie, Kathy Y (10)
|
SRBY |
39.00 |
NS |
- |
|
Hoffman, Ellie G (10)
|
ORLOSC |
50.11 |
NS |
- |