| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vecchio, Samantha E (8)
|
NIEMAC |
50.90 |
47.91 |
-2.99 |
| 2 |
Bradt, Addison M (8)
|
NIGVST |
45.55 |
48.11 |
2.56 |
| 3 |
Landow, Kayci L (7)
|
NIFKVL |
47.77 |
49.26 |
1.49 |
| 4 |
Green, Ella A (8)
|
NIGVST |
48.18 |
49.51 |
1.33 |
| 5 |
Budinger, Annabelle R (8)
|
NIGVST |
52.00 |
57.96 |
5.96 |
| 6 |
Sullivan, Mya M (7)
|
NIFKVL |
54.51 |
1:00.30 |
5.79 |
| 7 |
Owens, Annabelle R (7)
|
NIFKVL |
58.53 |
1:03.06 |
4.53 |
| 8 |
Graham, Gracyn M (7)
|
NIGVST |
1:05.53 |
1:04.67 |
-0.86 |
| 9 |
Castellano, Rebecca J (7)
|
NIGVST |
1:05.19 |
1:07.11 |
1.92 |
| 10 |
Dougherty, Regina A (7)
|
NIGVST |
1:03.77 |
1:08.82 |
5.05 |
| 11 |
Slocum, Molly M (7)
|
NIAAAC |
1:11.94 |
1:10.47 |
-1.47 |
| 12 |
Scott, Madelynn G (7)
|
NIGVST |
1:17.41 |
1:12.73 |
-4.68 |
| 13 |
Ellison, Paige A (7)
|
NIEMAC |
1:08.32 |
1:12.76 |
4.44 |
| 14 |
Hunt, Camdyn I (7)
|
NIGVST |
1:22.85 |
1:13.83 |
-9.02 |
| 15 |
Vossler, Rowan K (7)
|
NIGVST |
1:32.34 |
1:28.84 |
-3.50 |
|
Hirsch, McKenna L (6)
|
NIAAAC |
1:21.48 |
DQ |
- |
|
Bush, Zoey M (8)
|
NIEMAC |
51.03 |
NS |
- |
|
Bean, Mia G (8)
|
NIEMAC |
1:03.58 |
NS |
- |