| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cooper, Addison L (10)
|
MAPAY |
35.43 |
34.14 |
-1.29 |
| 2 |
Smallwood, Charlotte R (10)
|
MARKRY |
36.95 |
36.62 |
-0.33 |
| 2 |
Roulstone, Samantha J (9)
|
MAPAY |
37.46 |
36.62 |
-0.84 |
| 4 |
Yuan, Carolyn Y (9)
|
MATHY |
40.00 |
38.07 |
-1.93 |
| 5 |
Truong, Lauren (10)
|
MAPAY |
41.46 |
39.59 |
-1.87 |
| 6 |
O'Donnell, Morgan R (10)
|
MARKRY |
42.23 |
40.13 |
-2.10 |
| 7 |
Reiner, Ema D (10)
|
MARAYS |
|
40.56 |
- |
| 8 |
Hummel, Meredith D (9)
|
MAPAY |
55.99 |
40.62 |
-15.37 |
| 9 |
Matti, Abigail (10)
|
MDYCM |
43.53 |
40.73 |
-2.80 |
| 10 |
Knouft, Keira A (10)
|
MAUMLY |
46.87 |
40.92 |
-5.95 |
| 11 |
Flores-Caceres, Genesis G (9)
|
MAWEST |
39.21 |
40.95 |
1.74 |
| 12 |
Holland, Grace (10)
|
MAWEST |
38.85 |
41.00 |
2.15 |
| 13 |
Randolph, Chloe M (10)
|
MAUMLY |
46.25 |
42.41 |
-3.84 |
| 14 |
Li, Belle (9)
|
MDYCM |
47.86 |
42.90 |
-4.96 |
| 15 |
Coggiano, Ana M (9)
|
MDYCM |
45.70 |
44.43 |
-1.27 |
| 16 |
Hirsz, Nicole (9)
|
MATHY |
52.35 |
44.95 |
-7.40 |
| 17 |
Frustaci, Olivia (9)
|
MACBFY |
45.07 |
45.14 |
0.07 |
| 18 |
Lutz, Chloe A (9)
|
MAUMLY |
|
45.17 |
- |
| 19 |
Hendrawan, Chiara (10)
|
MACBFY |
44.45 |
45.38 |
0.93 |
| 20 |
Chen, Claire (9)
|
MAUMLY |
|
45.46 |
- |
| 21 |
Gaddameedi, Dhruti (9)
|
MAUMLY |
|
46.21 |
- |
| 22 |
Singh, Aanya (10)
|
MAPAY |
49.99 |
47.46 |
-2.53 |
| 23 |
Hirsz, Sofia (9)
|
MATHY |
52.38 |
48.87 |
-3.51 |
| 24 |
Esposito, Caitlyn A (9)
|
MDYCM |
54.50 |
49.43 |
-5.07 |
| 25 |
Anantharam, Maya P (9)
|
MDYCM |
54.47 |
51.73 |
-2.74 |
| 26 |
Cicero, Ryann E (8)
|
MAWCAY |
52.69 |
53.70 |
1.01 |
| 27 |
Prakash, Aditi (9)
|
MAPAY |
57.67 |
56.35 |
-1.32 |
| 28 |
Halloran, Corrine M (8)
|
MAWCAY |
58.34 |
1:06.89 |
8.55 |
| 29 |
Varani, Tessa P (7)
|
MATHY |
1:09.50 |
1:10.76 |
1.26 |