| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roy, Sylvia M (12)
|
AMMLAC |
6:21.20 |
5:47.69 |
-33.51 |
| 2 |
Shafer, Riley A (11)
|
AMPRA |
6:15.88 |
5:58.98 |
-16.90 |
| 3 |
Drew, Abigail E (12)
|
AMUN-M |
6:25.08 |
6:08.77 |
-16.31 |
| 4 |
Schomaker, Allison H (11)
|
AMPRA |
6:29.70 |
6:11.85 |
-17.85 |
| 5 |
Liu, Stacy R (11)
|
AMPTSC |
6:35.36 |
6:14.49 |
-20.87 |
| 6 |
Poe, Abby S (12)
|
AMPEAQ |
6:31.58 |
6:14.91 |
-16.67 |
| 7 |
Olson, Catherine (12)
|
AMPTSC |
6:39.42 |
6:28.34 |
-11.08 |
| 8 |
Crews, Abigail B (12)
|
AMPTSC |
6:48.32 |
6:32.33 |
-15.99 |
| 9 |
Bowers, Addison R (12)
|
AMPTSC |
6:48.94 |
6:35.50 |
-13.44 |
| 10 |
Dudkowski, Abigail E (12)
|
AMPRA |
6:44.30 |
6:44.53 |
0.23 |
| 11 |
Pearson, Lucy P (11)
|
AMPEAQ |
6:49.96 |
6:44.68 |
-5.28 |
| 12 |
Berg, Camille R (11)
|
AMSFAC |
6:57.25 |
6:46.72 |
-10.53 |
|
Koerner, Macy A (12)
|
AMPEAQ |
6:39.99 |
DQ |
- |