| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clodfelter, Claire L (12)
|
AMPEAQ |
3:21.79 |
3:10.87 |
-10.92 |
| 2 |
Shellenberger, Clara P (12)
|
AMMLAC |
3:20.33 |
3:12.51 |
-7.82 |
| 3 |
Kane, Maiya G (12)
|
AMPEAQ |
3:12.21 |
3:16.76 |
4.55 |
| 4 |
Clark, Makenzie E (12)
|
AMSVSC |
3:22.11 |
3:28.05 |
5.94 |
| 5 |
Recker, Reese M (11)
|
AMBPR |
3:40.00 |
3:29.06 |
-10.94 |
| 6 |
Wilson, Lana M (11)
|
AMRXA |
3:20.99 |
3:29.42 |
8.43 |
| 7 |
Eichelberger, Isabella K (12)
|
AMNAC |
3:40.96 |
3:36.35 |
-4.61 |
| 8 |
Muralidharan, Risha S (12)
|
AMSTEE |
3:41.17 |
3:41.13 |
-0.04 |
| 9 |
Molinero, Adalynn G (12)
|
AMSVSC |
3:37.99 |
3:41.40 |
3.41 |
| 10 |
Davis, Nora K (11)
|
AMNAC |
3:31.30 |
3:43.51 |
12.21 |
| 11 |
Morgan, Samantha A (11)
|
AMNAC |
3:33.54 |
3:44.89 |
11.35 |
|
Peretti, Cameron S (12)
|
AMWHAT |
3:21.15 |
NS |
- |