| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Teter, Jacob M (16)
|
OHPHST |
59.52 |
1:00.14 |
0.62 |
| 2 |
May, Caden M (15)
|
OHPHST |
1:02.02 |
1:01.87 |
-0.15 |
| 3 |
Masters, Austin M (16)
|
OHPHST |
1:01.45 |
1:02.18 |
0.73 |
| 4 |
Morse, Olivia E (16)
|
OHUAC |
1:05.05 |
1:06.83 |
1.78 |
| 5 |
King, Grace E (16)
|
OHHURY |
1:07.53 |
1:07.75 |
0.22 |
| 6 |
Baillargeon, Dakotta T (16)
|
OHPHST |
1:12.47 |
1:09.65 |
-2.82 |
| 7 |
Roche, Kyleigh M (16)
|
OHDCST |
|
1:09.84 |
- |
| 8 |
Erickson, Macy M (15)
|
OHPHST |
1:11.34 |
1:10.90 |
-0.44 |
| 9 |
Madry, Jordan A (17)
|
OHUAC |
|
1:13.26 |
- |
| 10 |
Allen, Travis C (15)
|
OHPHST |
1:20.75 |
1:17.39 |
-3.36 |
|
Vanderzwan, Abigail M (17)
|
OHUAC |
1:51.10 |
NS |
- |