| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Neville, Philip D (12)
|
NICAGO |
36.66 |
36.07 |
-0.59 |
| 2 |
Webster, Hayne W (12)
|
NIUN |
38.00 |
38.16 |
0.16 |
| 3 |
Gorman, William G (12)
|
NIUNAC |
38.57 |
38.31 |
-0.26 |
| 4 |
Butler, Daine M (12)
|
NIGGST |
39.99 |
40.63 |
0.64 |
| 5 |
Gorsline, Kaden P (12)
|
NIWAAC |
41.39 |
41.58 |
0.19 |
| 6 |
Clarner, Benjamin A (12)
|
NICAGO |
40.91 |
41.81 |
0.90 |
| 7 |
Williams, Oscar B (11)
|
NIWAAC |
46.91 |
43.02 |
-3.89 |
| 8 |
McGovern, Patrick F (11)
|
NIUNAC |
46.14 |
43.59 |
-2.55 |
| 9 |
Beers, Keaton W (12)
|
NIWAAC |
43.68 |
44.78 |
1.10 |
| 10 |
Webster, Travis J (11)
|
NIWAAC |
48.16 |
44.95 |
-3.21 |
| 11 |
Ottoson, Trevor T (12)
|
ADSIDN |
|
48.64 |
- |
| 12 |
Venkataswamy, Eshwar (12)
|
NIWAAC |
49.56 |
48.98 |
-0.58 |
| 13 |
Blood, Joseph B (12)
|
NIWAAC |
|
55.59 |
- |
|
Cleveland, Matthew J (12)
|
NIUNAC |
1:17.14 |
DQ |
- |