| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trumpower, Adayaleise L (7)
|
OHUN |
21.29 |
19.29 |
-2.00 |
| 2 |
Dyussemaliyeva, Laila R (8)
|
OHWOR |
20.60 |
19.36 |
-1.24 |
| 3 |
Rickel, Adryan L (7)
|
LEUN-C |
21.75 |
21.08 |
-0.67 |
| 4 |
Lopez, Jennifer * (8)
|
OHBGSC |
23.17 |
22.66 |
-0.51 |
| 5 |
Schmidt, Hazel R (8)
|
OHGTAC |
25.21 |
23.60 |
-1.61 |
| 6 |
Pedraza, Blayne E (7)
|
OHNAC |
27.30 |
24.13 |
-3.17 |
| 7 |
Rando, Maylie P (8)
|
OHSOS |
26.26 |
24.36 |
-1.90 |
| 8 |
Rhoades, Emilia M (8)
|
OHSOS |
24.53 |
24.74 |
0.21 |
| 9 |
Bourn, Julia (7)
|
OHWAUS |
26.97 |
24.85 |
-2.12 |
| 10 |
Shawver, Lillian K (8)
|
OHBGSC |
24.56 |
25.04 |
0.48 |
| 11 |
Donald, Jamie M (8)
|
OHBGSC |
23.26 |
25.18 |
1.92 |
| 12 |
Johnson, Jaylee P (7)
|
OHNAC |
26.63 |
25.24 |
-1.39 |
| 13 |
Garon, Hailey R (7)
|
OHBGSC |
|
26.71 |
- |
| 14 |
Speiser, Piper H (8)
|
OHNAC |
29.14 |
26.72 |
-2.42 |
| 15 |
Lim, Sharon Y (7)
|
OHSYL |
27.97 |
27.05 |
-0.92 |
| 16 |
Hernandez, Melody * (7)
|
OHBGSC |
29.70 |
27.47 |
-2.23 |
| 17 |
Alt, Eden E (8)
|
OHBGSC |
|
27.96 |
- |
| 18 |
Flowers, Emily M (8)
|
OHNAC |
32.79 |
29.50 |
-3.29 |
| 19 |
Eisenhauer, Brooklynn G (7)
|
OHSOS |
|
29.58 |
- |
| 20 |
Staton, Marley J (8)
|
OHNAC |
28.35 |
30.91 |
2.56 |
| 21 |
Misra, Kaliani R (6)
|
OHGTAC |
33.65 |
32.44 |
-1.21 |
| 22 |
Boehler, Chloye A (8)
|
OHBGSC |
|
35.06 |
- |
|
Knepley, Tessa L (7)
|
OHNAC |
27.87 |
DQ |
- |
|
Misra, Karmina R (5)
|
OHGTAC |
56.40 |
DQ |
- |