| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trumpower, Adayaleise L (7)
|
OHUN |
33.27 |
32.68 |
-0.59 |
| 2 |
Dyussemaliyeva, Laila R (8)
|
OHWOR |
39.05 |
37.48 |
-1.57 |
| 3 |
Rickel, Adryan L (7)
|
LEUN-C |
39.88 |
38.43 |
-1.45 |
| 4 |
Lopez, Jennifer * (8)
|
OHBGSC |
42.48 |
42.67 |
0.19 |
| 5 |
Donald, Jamie M (8)
|
OHBGSC |
42.88 |
43.83 |
0.95 |
| 5 |
Hildebrand, Leah P (8)
|
OHBGSC |
52.50 |
43.83 |
-8.67 |
| 7 |
Rhoades, Emilia M (8)
|
OHSOS |
43.80 |
44.68 |
0.88 |
| 8 |
Bourn, Julia (7)
|
OHWAUS |
|
46.09 |
- |
| 9 |
Rando, Maylie P (8)
|
OHSOS |
51.17 |
50.35 |
-0.82 |
| 10 |
Shawver, Lillian K (8)
|
OHBGSC |
42.09 |
51.10 |
9.01 |
| 11 |
Schmidt, Hazel R (8)
|
OHGTAC |
51.78 |
53.02 |
1.24 |
| 12 |
Garon, Hailey R (7)
|
OHBGSC |
|
53.14 |
- |
| 13 |
Lim, Sharon Y (7)
|
OHSYL |
|
57.15 |
- |
| 14 |
Alt, Eden E (8)
|
OHBGSC |
|
58.16 |
- |
| 15 |
Eisenhauer, Brooklynn G (7)
|
OHSOS |
|
1:05.32 |
- |
| 16 |
Hernandez, Melody * (7)
|
OHBGSC |
1:00.08 |
1:07.64 |
7.56 |
| 17 |
Misra, Kaliani R (6)
|
OHGTAC |
1:12.59 |
1:17.54 |
4.95 |
| 18 |
Misra, Karmina R (5)
|
OHGTAC |
3:15.70 |
1:47.49 |
-88.21 |
|
Boehler, Chloye A (8)
|
OHBGSC |
|
NS |
- |