| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Conrad, Brynn J (16)
|
OHUSA |
2:38.43 |
2:35.83 |
-2.60 |
| 2 |
Delma, Paige O (16)
|
OHWOR |
2:34.38 |
2:36.81 |
2.43 |
| 3 |
Gano, Haley N (16)
|
OHFMST |
2:35.48 |
2:38.29 |
2.81 |
| 4 |
Stith, Annaliess D (15)
|
OHGCST |
2:19.68 |
2:38.30 |
18.62 |
| 5 |
Carter, Mackenzie M (16)
|
OHGCST |
2:40.25 |
2:39.56 |
-0.69 |
| 6 |
Grespin, Margaret S (16)
|
OHGTAC |
2:37.41 |
2:41.24 |
3.83 |
| 7 |
Desmond, Madeline G (15)
|
OHGTAC |
2:46.84 |
2:41.34 |
-5.50 |
| 8 |
Recor, Elle A (16)
|
OHUSA |
2:45.19 |
2:41.62 |
-3.57 |
| 9 |
Neininger, Brooke I (15)
|
OHBKYS |
2:49.88 |
2:45.59 |
-4.29 |
| 10 |
Randall, Melaina B (15)
|
OHBKYS |
2:52.70 |
2:46.60 |
-6.10 |
| 11 |
Bell, Ava E (16)
|
OHGTAC |
2:47.07 |
2:47.60 |
0.53 |
| 12 |
Gaver, Araceli S (15)
|
OHGCST |
2:50.99 |
2:47.91 |
-3.08 |
| 13 |
Dyussemaliyeva, Diana R (15)
|
OHWOR |
2:52.34 |
2:48.78 |
-3.56 |
| 14 |
Walter, Ashlyn M (16)
|
OHDCST |
2:45.74 |
2:49.80 |
4.06 |
| 15 |
O'Brien, Emily M (15)
|
OHBGSC |
2:55.71 |
2:54.69 |
-1.02 |
| 16 |
Di-Nardo, Abbigail B (16)
|
OHEP |
2:45.13 |
2:54.97 |
9.84 |
| 17 |
Layne, Margaret S (15)
|
OHBGSC |
2:59.63 |
2:55.16 |
-4.47 |
| 18 |
Peltcs, Avery M (15)
|
OHUSA |
2:49.75 |
2:55.58 |
5.83 |
| 19 |
Tennant, Mia E (15)
|
OHSYL |
3:00.59 |
2:56.18 |
-4.41 |
| 20 |
Hiller, Anna (15)
|
OHUSA |
3:04.90 |
2:57.33 |
-7.57 |
| 21 |
Neese, Chayse J (16)
|
OHGCST |
3:10.96 |
3:04.53 |
-6.43 |