| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vann, Reilly P (16)
|
OHUSA |
2:23.51 |
2:22.20 |
-1.31 |
| 2 |
Yates, Blake W (16)
|
OHEP |
2:11.16 |
2:22.31 |
11.15 |
| 3 |
Van Cott, Joshua T (16)
|
OHSYL |
2:21.71 |
2:23.80 |
2.09 |
| 4 |
Gorobchuk, Yuriy (16)
|
OHUSA |
2:28.04 |
2:24.67 |
-3.37 |
| 5 |
Rabadam, Jared M (15)
|
OHUSA |
2:31.29 |
2:26.85 |
-4.44 |
| 6 |
Harpster, William J (15)
|
OHGCST |
2:31.79 |
2:28.15 |
-3.64 |
| 7 |
Gaier, Michael S (15)
|
OHLTPY |
2:34.30 |
2:28.77 |
-5.53 |
| 8 |
Keller, Jackson P (16)
|
OHWOR |
2:24.05 |
2:29.04 |
4.99 |
| 10 |
Lewis, Gavin P (16)
|
OHGCST |
2:11.59 |
2:30.53 |
18.94 |
| 11 |
Rumburg, Noah P (15)
|
OHLTPY |
2:30.20 |
2:31.31 |
1.11 |
| 12 |
Fisher, Ewan C (15)
|
OHBGSC |
2:32.92 |
2:31.66 |
-1.26 |
| 13 |
Ambler, Benjamin M (16)
|
OHWOR |
2:37.24 |
2:32.43 |
-4.81 |
| 14 |
Moore, Tyler D (16)
|
OHUSA |
2:37.62 |
2:33.01 |
-4.61 |
| 15 |
Harman, Quinn R (16)
|
OHGCST |
2:57.30 |
2:33.30 |
-24.00 |
| 16 |
Randolph, Jayden M (16)
|
OHDCST |
2:30.30 |
2:34.95 |
4.65 |
| 17 |
Ambler, Nathan J (16)
|
OHWOR |
2:34.55 |
2:35.43 |
0.88 |
| 18 |
Zoldowski, Nicholas J (16)
|
OHGTAC |
2:34.80 |
2:35.64 |
0.84 |
| 19 |
He, Aaron W (15)
|
OHBGSC |
2:35.76 |
2:36.07 |
0.31 |
| 20 |
Stottmann, Matthew R (15)
|
OHUBKY |
2:24.67 |
2:39.98 |
15.31 |
| 21 |
Wickham, Cooper (15)
|
OHUSA |
2:43.92 |
2:41.74 |
-2.18 |
| 22 |
Merriman, Seth E (15)
|
OHFMST |
2:39.98 |
2:43.78 |
3.80 |
| 23 |
Miller, Mason W (15)
|
OHLTPY |
2:28.97 |
2:44.13 |
15.16 |
| 24 |
Hou, Peter (15)
|
OHWOR |
2:56.80 |
2:46.83 |
-9.97 |
| 25 |
Mejiritski, Aaron J (16)
|
OHBGSC |
2:54.67 |
2:48.45 |
-6.22 |
| 26 |
Jones, Dexter D (15)
|
OHWOR |
3:23.64 |
2:49.19 |
-34.45 |
| 27 |
Price, Jasper C (16)
|
OHBGSC |
2:56.47 |
2:57.91 |
1.44 |