| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Racke, Christina K (15)
|
OHLTPY |
2:32.63 |
2:32.78 |
0.15 |
| 2 |
Hofmeister, Morgan R (16)
|
OHUSA |
2:43.19 |
2:34.50 |
-8.69 |
| 3 |
Gano, Haley N (16)
|
OHFMST |
2:29.26 |
2:34.89 |
5.63 |
| 4 |
Cicak, Isabella (15)
|
OHDCST |
2:41.16 |
2:35.64 |
-5.52 |
| 5 |
Boone-McDougall, Sydney E (15)
|
OHDCST |
2:38.21 |
2:38.87 |
0.66 |
| 6 |
Desmond, Madeline G (15)
|
OHGTAC |
2:43.94 |
2:39.57 |
-4.37 |
| 7 |
Kinzeler, Lauren A (16)
|
OHKCST |
2:34.33 |
2:40.11 |
5.78 |
| 8 |
Palmer, Anila M (15)
|
OHGCST |
2:51.01 |
2:42.34 |
-8.67 |
| 9 |
Dyussemaliyeva, Diana R (15)
|
OHWOR |
2:47.73 |
2:42.52 |
-5.21 |
| 10 |
Blakely, Amelie C (16)
|
OHBGSC |
2:45.79 |
2:43.97 |
-1.82 |
| 11 |
Bell, Ava E (16)
|
OHGTAC |
2:42.66 |
2:45.96 |
3.30 |
| 12 |
Musteric, Mary E (15)
|
OHBGSC |
2:43.70 |
2:47.36 |
3.66 |
| 13 |
Weissling, Ava N (15)
|
OHBGSC |
2:54.64 |
2:48.32 |
-6.32 |
| 14 |
Gaver, Araceli S (15)
|
OHGCST |
2:53.16 |
2:50.31 |
-2.85 |
| 15 |
DiSalle, Vivian E (16)
|
OHGTAC |
2:58.15 |
2:51.00 |
-7.15 |
| 16 |
Li, Alysa D (15)
|
OHGCST |
2:52.78 |
2:54.22 |
1.44 |
| 17 |
Peltcs, Avery M (15)
|
OHUSA |
2:56.95 |
2:58.98 |
2.03 |
| 18 |
Layne, Margaret S (15)
|
OHBGSC |
3:01.71 |
3:01.00 |
-0.71 |
| 19 |
Hiller, Anna (15)
|
OHUSA |
3:06.09 |
3:05.09 |
-1.00 |
|
Freeman, Fiona E (15)
|
OHBGSC |
3:03.24 |
DQ |
- |