| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Weaver, Maryn M (10)
|
OHBKYS |
44.17 |
44.22 |
0.05 |
| 2 |
Syrek, Ashlyn O (10)
|
OHFMST |
45.92 |
46.15 |
0.23 |
| 3 |
Ransom, Jessie E (10)
|
OHBKYS |
47.43 |
48.08 |
0.65 |
| 4 |
Dyussemaliyeva, Laila R (10)
|
OHWOR |
50.47 |
48.58 |
-1.89 |
| 5 |
Wu, Noel K (10)
|
OHBKYS |
50.26 |
48.84 |
-1.42 |
| 6 |
Starner, Zoey M (10)
|
OHUSA |
48.70 |
49.50 |
0.80 |
| 7 |
Drees, Joy C (9)
|
OHGTAC |
51.43 |
49.56 |
-1.87 |
| 8 |
Smith, Peighton H (9)
|
OHBKYS |
49.74 |
49.77 |
0.03 |
| 9 |
Schrimpf, Miriam G (10)
|
OHGCST |
44.45 |
49.89 |
5.44 |
| 10 |
Geskey, Avery A (9)
|
OHUSA |
47.71 |
49.99 |
2.28 |
| 11 |
Stannert, Lillian G (10)
|
OHFMST |
50.69 |
51.29 |
0.60 |
| 12 |
Lindhorst, Charlotte C (10)
|
OHGTAC |
55.74 |
51.37 |
-4.37 |
| 13 |
Ehret, Charlotte L (10)
|
OHDCST |
53.07 |
51.76 |
-1.31 |
| 14 |
Bigelow, Emmerson G (10)
|
OHFMST |
52.49 |
52.78 |
0.29 |
| 15 |
Drees, Jasmine L (9)
|
OHGTAC |
53.82 |
53.01 |
-0.81 |
| 16 |
Marshall, Eloise G (10)
|
OHGTAC |
55.67 |
54.21 |
-1.46 |
| 17 |
Wittmer, Marin A (8)
|
OHFMST |
57.57 |
54.77 |
-2.80 |
| 18 |
Schlachter, Lily R (10)
|
OHSYL |
1:00.49 |
56.92 |
-3.57 |
| 19 |
Moellenberg, Emily S (10)
|
OHSYL |
1:11.35 |
56.96 |
-14.39 |
| 20 |
Pyles, Emma J (9)
|
OHUSA |
1:04.86 |
1:01.72 |
-3.14 |
| 21 |
Newell, Raelynn S (10)
|
OHEP |
55.21 |
1:03.96 |
8.75 |
| 22 |
Lester, Sara R (8)
|
OHBKYS |
1:07.30 |
1:07.36 |
0.06 |
| 23 |
McDonald, Penny J (7)
|
OHBKYS |
1:17.11 |
1:16.54 |
-0.57 |
|
Alisiswanto, Zoe E (7)
|
OHBKYS |
1:07.65 |
DQ |
- |