| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Trumpower, Grantham J (12)
|
OHUN |
56.07 |
55.74 |
-0.33 |
| 2 |
Ball, Wyatt A (12)
|
OHFAST |
58.57 |
58.39 |
-0.18 |
| 3 |
Al-Akhras, Yusuf K (12)
|
OHGTAC |
1:01.54 |
1:02.14 |
0.60 |
| 4 |
Crooks, Maxwell M (12)
|
OHSYL |
1:03.01 |
1:02.57 |
-0.44 |
| 5 |
Sorg, Austin S (12)
|
OHRSC |
1:01.60 |
1:02.89 |
1.29 |
| 6 |
Dietrich, Tobias J (12)
|
OHSYL |
1:05.51 |
1:05.06 |
-0.45 |
| 7 |
Mowery, Brayden C (12)
|
OHGTAC |
1:05.45 |
1:07.09 |
1.64 |
| 8 |
Guo, Richard R (12)
|
OHSYL |
1:09.67 |
1:07.24 |
-2.43 |
| 9 |
O'Brien, Aiden J (12)
|
OHBGSC |
1:08.99 |
1:07.53 |
-1.46 |
| 10 |
Pelz, Cody W (11)
|
OHRSC |
1:09.71 |
1:08.08 |
-1.63 |
| 11 |
Gerken, Reed W (11)
|
OHFMST |
1:09.78 |
1:09.17 |
-0.61 |
| 12 |
Ament, Logan J (11)
|
OHWOR |
1:04.77 |
1:09.58 |
4.81 |
| 13 |
Florence, Cooper E (11)
|
OHWOR |
1:08.35 |
1:11.03 |
2.68 |
| 14 |
Sattig, Fynn E (11)
|
OHFMST |
1:10.35 |
1:11.33 |
0.98 |
| 15 |
Dyussemaliyev, Almas R (12)
|
OHWOR |
1:10.31 |
1:11.59 |
1.28 |
| 16 |
O'Neill, Brennan J (12)
|
OHRSC |
1:12.12 |
1:12.52 |
0.40 |
| 17 |
Montgomery, Cole J (11)
|
OHWOR |
1:10.59 |
1:13.01 |
2.42 |
| 18 |
Flowers, Nick B (12)
|
OHNAC |
1:16.71 |
1:14.51 |
-2.20 |
| 19 |
Poland, Nathaniel P (11)
|
OHFMST |
1:12.23 |
1:15.57 |
3.34 |
| 20 |
Sturdevant, Zane L (11)
|
OHGTAC |
1:07.58 |
1:17.32 |
9.74 |
| 21 |
Pena, Tristan J (11)
|
OHWAUS |
1:14.76 |
1:17.72 |
2.96 |
| 22 |
Mierzejewski, Drew * (12)
|
OHBGSC |
|
1:22.09 |
- |
| 23 |
Colon, Miles (11)
|
OHWAUS |
1:56.16 |
1:23.65 |
-32.51 |
| 24 |
Wasnich, Clay (11)
|
OHWAUS |
1:39.15 |
1:24.99 |
-14.16 |
| 25 |
Barnhart, Brayden B (11)
|
OHBGSC |
1:23.67 |
1:28.26 |
4.59 |
| 26 |
Best, Jacob M (11)
|
OHFAST |
1:40.56 |
1:29.38 |
-11.18 |
| 27 |
Van Vorhis, Kaleb R (11)
|
OHBGSC |
1:27.99 |
1:33.94 |
5.95 |
| 28 |
Yang, Joseph H (11)
|
OHSYL |
1:32.08 |
1:39.73 |
7.65 |
|
Allred, Jasper D (11)
|
OHGTAC |
2:09.36 |
NS |
- |
|
Harman, Landon C (12)
|
OHSYL |
1:08.96 |
NS |
- |