| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dyussemaliyeva, Laila R (8)
|
OHWOR |
51.36 |
51.32 |
-0.04 |
| 2 |
Bigelow, Emmerson G (8)
|
OHFMST |
56.36 |
55.21 |
-1.15 |
| 3 |
Stannert, Lillian G (8)
|
OHFMST |
55.94 |
57.19 |
1.25 |
| 4 |
Castrillon-Gomez, Sofia (7)
|
OHFMST |
56.00 |
59.29 |
3.29 |
| 5 |
Rhoades, Emilia M (8)
|
OHSOS |
1:00.85 |
1:03.03 |
2.18 |
| 6 |
Shawver, Lillian K (8)
|
OHBGSC |
58.94 |
1:06.11 |
7.17 |
| 7 |
Rando, Maylie P (8)
|
OHSOS |
1:00.00 |
1:14.95 |
14.95 |
| 8 |
Lim, Sharon Y (7)
|
OHSYL |
|
1:23.84 |
- |
| 9 |
Sunday, Alaina J (7)
|
OHFMST |
|
1:27.60 |
- |
|
Alt, Eden E (8)
|
OHBGSC |
|
DQ |
- |
|
Garon, Hailey R (7)
|
OHBGSC |
|
DQ |
- |
|
Lopez, Jennifer * (8)
|
OHBGSC |
|
DQ |
- |
|
Kessler, Kieradyn M (8)
|
OHFMST |
|
DQ |
- |
|
Trumpower, Adayaleise L (7)
|
OHUN |
46.60 |
DQ |
- |