| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Majumdar, Ryka (10)
|
OHWOR |
40.11 |
41.31 |
1.20 |
| 2 |
Syrek, Annabel K (9)
|
OHFMST |
49.20 |
45.66 |
-3.54 |
| 3 |
Syrek, Ashlyn O (9)
|
OHFMST |
46.60 |
46.47 |
-0.13 |
| 4 |
Konieczka, Hayden P (10)
|
OHWAUS |
49.25 |
46.87 |
-2.38 |
| 5 |
Wojciechowski, Harper M (10)
|
OHFMST |
49.15 |
47.88 |
-1.27 |
| 6 |
Packo, Annie J (10)
|
OHGTAC |
46.25 |
47.89 |
1.64 |
| 7 |
Baker, Hayley J (10)
|
OHBGSC |
48.05 |
49.15 |
1.10 |
| 8 |
Zimmerman, Gabrielle L (10)
|
OHWOR |
47.47 |
49.23 |
1.76 |
| 9 |
Cirman, Elena M (10)
|
OHWOR |
48.04 |
50.20 |
2.16 |
| 10 |
Okoneski, Ella C (10)
|
OHDORC |
53.55 |
50.50 |
-3.05 |
| 11 |
Schrader, Ivy E (10)
|
OHBGSC |
1:00.77 |
51.39 |
-9.38 |
| 12 |
Sunday, Ava R (10)
|
OHFMST |
50.75 |
51.72 |
0.97 |
| 13 |
Nixon, Natalie P (9)
|
OHGTAC |
51.07 |
52.32 |
1.25 |
| 14 |
Bublick, Quinn A (9)
|
OHFMST |
51.59 |
52.60 |
1.01 |
| 15 |
Randecker, Sydney G (9)
|
OHFMST |
53.49 |
52.68 |
-0.81 |
| 16 |
Saksa, Claire A (9)
|
OHDORC |
|
55.62 |
- |
| 17 |
Rutherford, Raegan R (9)
|
OHSYL |
56.41 |
57.99 |
1.58 |
| 18 |
Carroll, Bristol T (9)
|
OHFMST |
56.08 |
58.58 |
2.50 |
| 19 |
Cline, Meara E (9)
|
OHWOR |
1:01.03 |
1:00.87 |
-0.16 |
| 20 |
Hufford, Evie G (9)
|
OHWOR |
1:03.85 |
1:07.15 |
3.30 |
|
Brink, Chloe M (9)
|
OHNAC |
|
DQ |
- |
|
Harman, Emerson J (9)
|
OHSYL |
1:01.26 |
NS |
- |
|
Rocha, Bentlei (9)
|
OHWAUS |
1:16.69 |
NS |
- |