| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Brooks, Heidi J (16)
|
KYUN |
2:33.90 |
2:31.75 |
-2.15 |
| 2 |
Zinobile, Allison C (18)
|
KYRACE |
2:21.34 |
2:36.24 |
14.90 |
| 3 |
Noyes, Penelope P (65)
|
KYSKY |
2:45.67 |
2:36.84 |
-8.83 |
| 4 |
Rigsby-Tinch, Frannie (15)
|
KYSKY |
2:31.60 |
2:44.04 |
12.44 |
| 5 |
Witcher, Leah F (16)
|
KYRACE |
2:37.87 |
2:46.23 |
8.36 |
| 6 |
Wheeler, Carolina G (14)
|
KYRACE |
2:35.31 |
2:46.38 |
11.07 |
| 7 |
Brooks, McKinley A (13)
|
KYUN |
|
2:49.70 |
- |
| 8 |
Barbee, Madeline E (14)
|
KYRACE |
|
2:54.31 |
- |
| 9 |
Steelman, Peyton R (15)
|
KYRACE |
2:53.28 |
3:02.25 |
8.97 |
| 10 |
Matzke, Amelia K (13)
|
KYRACE |
|
3:23.14 |
- |
| 11 |
Willett, Carolyn G (13)
|
KYRACE |
3:19.43 |
3:24.16 |
4.73 |
| 12 |
Calloway, Caroline B (13)
|
KYRACE |
3:09.91 |
3:33.28 |
23.37 |