| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mohlenbrok, Gabriel (17)
|
MRMPNY |
52.71 |
50.81 |
-1.90 |
| 1 |
Mercado, David (18)
|
MRUNLS |
52.79 |
52.10 |
-0.69 |
| 2 |
Hernandez, Luis (16)
|
MRCAS |
54.09 |
51.63 |
-2.46 |
| 2 |
Vaynshteyn, Gabriel (15)
|
MRMPNY |
52.26 |
52.65 |
0.39 |
| 3 |
Arkhangelskiy, Michael (15)
|
MRMPNY |
53.33 |
52.70 |
-0.63 |
| 3 |
Green, Ryan L (15)
|
MRYFD |
54.19 |
54.50 |
0.31 |
| 4 |
Hadary, Daniel B (15)
|
MRFLY |
54.01 |
52.94 |
-1.07 |
| 4 |
Prokic, Matija (15)
|
MRRIST |
54.48 |
54.73 |
0.25 |
| 5 |
Kourilov, Yevgeniy (15)
|
MRMPNY |
53.46 |
53.49 |
0.03 |
| 5 |
Haque, Nayem (18)
|
MRKBM |
54.48 |
54.98 |
0.50 |
| 6 |
Cusack-McPartlin, Timothy (16)
|
MRWAC |
54.21 |
55.58 |
1.37 |
| 7 |
Gutierrez, Oman M (15)
|
MRKBM |
54.09 |
56.27 |
2.18 |
|
Pan, Matthew A (16)
|
MRGATE |
53.64 |
NS |
- |
|
Drallios, Konstantin (16)
|
MRLGAC |
53.07 |
NS |
- |