| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Franke, Hunter J (12)
|
WIOCSC |
48.13 |
45.64 |
-2.49 |
| 2 |
Mrotek, Nolan J (12)
|
WISEA |
46.74 |
46.42 |
-0.32 |
| 3 |
Stahl, Brody D (11)
|
ILFVFY |
48.71 |
46.85 |
-1.86 |
| 4 |
Torres, Aidan E (11)
|
ILFVFY |
52.26 |
49.49 |
-2.77 |
| 5 |
Bartels, Grant R (11)
|
WIMM |
55.62 |
51.73 |
-3.89 |
| 6 |
Schmitt, Landen R (11)
|
WIFAST |
51.42 |
52.30 |
0.88 |
| 7 |
Foster, Nathaniel E (12)
|
WISEA |
53.23 |
53.35 |
0.12 |
| 8 |
Spleha, Christopher M (12)
|
ILFVFY |
|
53.47 |
- |
| 9 |
Lippoldt, Owen O (11)
|
ILFVFY |
56.60 |
54.77 |
-1.83 |
| 10 |
Trajcev, Aleksandar A (12)
|
WINBSC |
|
58.86 |
- |
| 11 |
Woletz, Keaton J (11)
|
WIMM |
1:03.75 |
1:04.93 |
1.18 |
|
Framberg, Nolan A (11)
|
WISEA |
|
DQ |
- |
|
Benson, Connor J (11)
|
WIMM |
|
NS |
- |
|
Borzynski, Jack M (11)
|
WISEA |
49.10 |
NS |
- |