| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Franke, Hunter J (12)
|
WIOCSC |
32.85 |
33.08 |
0.23 |
| 2 |
Torres, Aidan E (11)
|
ILFVFY |
35.14 |
33.54 |
-1.60 |
| 3 |
Schmitt, Landen R (11)
|
WIFAST |
35.17 |
34.14 |
-1.03 |
| 4 |
Lippoldt, Owen O (11)
|
ILFVFY |
37.73 |
34.55 |
-3.18 |
| 5 |
Foster, Nathaniel E (12)
|
WISEA |
35.87 |
37.20 |
1.33 |
| 6 |
Spleha, Christopher M (12)
|
ILFVFY |
|
37.59 |
- |
| 7 |
Stahl, Brody D (11)
|
ILFVFY |
39.61 |
37.67 |
-1.94 |
| 8 |
Bartels, Grant R (11)
|
WIMM |
39.94 |
37.99 |
-1.95 |
| 9 |
Holmes, Jayden L (11)
|
WIMM |
44.35 |
39.73 |
-4.62 |
| 10 |
Woletz, Keaton J (11)
|
WIMM |
41.74 |
42.46 |
0.72 |
| 11 |
Trajcev, Aleksandar A (12)
|
WINBSC |
|
44.04 |
- |
| 12 |
Moesch, Ashton J (12)
|
WISEA |
56.81 |
51.94 |
-4.87 |
|
Hawkins, Landon B (11)
|
ILFVFY |
|
DQ |
- |
|
Benson, Connor J (11)
|
WIMM |
|
NS |
- |
|
Framberg, Nolan A (11)
|
WISEA |
|
NS |
- |
|
Moore, Brady J (12)
|
WISEA |
|
NS |
- |