| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Schultz, Grace M (14)
|
WIWYNS |
57.30 |
57.05 |
-0.25 |
| 2 |
Lemler, Emma J (13)
|
WISSTY |
1:00.00 |
59.22 |
-0.78 |
| 3 |
Erstad, Morgan M (14)
|
WIGCY |
58.00 |
1:00.30 |
2.30 |
| 4 |
Whowell, Sailor W (13)
|
WISSTY |
58.91 |
1:00.77 |
1.86 |
| 5 |
Flanagan, Grace R (14)
|
WIFCY |
1:00.63 |
1:00.86 |
0.23 |
| 6 |
Zeinert, Melana A (14)
|
WIOSHY |
1:01.48 |
1:01.38 |
-0.10 |
| 7 |
Badillo, Sofia (13)
|
WISEA |
1:02.04 |
1:01.64 |
-0.40 |
| 8 |
Peterson, Molli B (14)
|
WIOSHY |
1:02.39 |
1:01.81 |
-0.58 |
| 9 |
Roelli, Alyssa M (14)
|
WIGCY |
1:01.69 |
1:02.45 |
0.76 |
| 10 |
Doczy, Isabel M (14)
|
WISSTY |
1:02.77 |
1:02.68 |
-0.09 |
| 11 |
Raczek, Isabella M (13)
|
WISPDY |
1:02.90 |
1:03.18 |
0.28 |
| 12 |
Osthelder, Ellen S (13)
|
WIYDC |
1:04.15 |
1:04.56 |
0.41 |
| 13 |
Brost, Kallie J (14)
|
WIWYNS |
1:05.97 |
1:04.69 |
-1.28 |
| 14 |
Orzech, Bailey S (14)
|
WIWYNS |
1:06.03 |
1:05.51 |
-0.52 |
| 15 |
Neville, Danica M (13)
|
WIDCY |
1:06.68 |
1:05.71 |
-0.97 |
| 16 |
Wenszell, Hannah E (13)
|
WISSTY |
1:06.37 |
1:06.10 |
-0.27 |
| 17 |
Michalak, Tegan (13)
|
WILAXY |
1:08.21 |
1:06.64 |
-1.57 |
| 18 |
Topritzhofer, Annika E (13)
|
WICVY |
1:07.63 |
1:06.67 |
-0.96 |
| 19 |
Hill, Gracielinn (14)
|
WIDCY |
1:06.51 |
1:06.68 |
0.17 |
| 20 |
Dietschweiler, Kaitlyn F (13)
|
WIOSHY |
1:08.23 |
1:06.87 |
-1.36 |
| 21 |
Cleveland, Libby (13)
|
WISCY |
1:05.68 |
1:07.16 |
1.48 |
| 22 |
Scott, Samantha B (14)
|
DCNLY |
1:05.91 |
1:07.54 |
1.63 |
| 23 |
Baumbich, Bridget A (13)
|
WILAXY |
1:08.05 |
1:08.67 |
0.62 |
| 24 |
Gutierrez-Jakobi, Michelle (14)
|
WIWYNS |
1:11.30 |
1:08.84 |
-2.46 |
| 25 |
Pavelchik, Portia S (13)
|
WISSTY |
1:06.76 |
1:09.38 |
2.62 |
| 26 |
Voeks, Rowan J (13)
|
WIYDC |
1:12.08 |
1:11.22 |
-0.86 |
| 27 |
Scherf, Delaney A (13)
|
WIECY |
1:10.75 |
1:13.36 |
2.61 |
| 28 |
Heinz, Ava (13)
|
WICVY |
1:10.55 |
1:13.97 |
3.42 |
| 29 |
Archibald-Smith, Paige G (13)
|
WIECY |
1:14.01 |
1:15.01 |
1.00 |