| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wen, Dai Gao (14)
|
NIUN-D |
33.11 |
31.16 |
-1.95 |
| 2 |
Pierce, Nicolin R (14)
|
AMMIST |
34.25 |
33.17 |
-1.08 |
| 3 |
Blomberg, Mark (14)
|
NIUN-D |
34.00 |
33.68 |
-0.32 |
| 4 |
Monteiro, Phillip G (14)
|
NIVELO |
34.58 |
34.21 |
-0.37 |
| 5 |
Klein, Adam C (14)
|
NIVELO |
36.97 |
36.07 |
-0.90 |
| 6 |
Currier, Mason L (13)
|
AMPWRS |
39.97 |
36.39 |
-3.58 |
| 7 |
Stockschlaeder, Herbert A (14)
|
NIHAMB |
37.56 |
36.45 |
-1.11 |
| 8 |
Khurshudyan, Yuriy S (13)
|
AMPWRS |
37.43 |
36.54 |
-0.89 |
| 9 |
Stutts, Shawn T (13)
|
AMPWRS |
43.20 |
36.72 |
-6.48 |
| 10 |
Avino, Evan A (13)
|
NISTAR |
45.60 |
38.19 |
-7.41 |
| 11 |
Schraufstetter, Evan J (13)
|
NITTSC |
40.34 |
38.28 |
-2.06 |
| 12 |
Patel, Ved J (13)
|
AMMIST |
|
40.82 |
- |
| 13 |
LaMoy, Hunter P (13)
|
NIRAYS |
48.69 |
44.35 |
-4.34 |
| 14 |
Lorenz, Robert D (14)
|
NITTSC |
1:14.42 |
49.17 |
-25.25 |
| 15 |
Kaus, Hayden G (13)
|
NIHAMB |
49.24 |
50.73 |
1.49 |
|
McLaughlin, Patrick S (13)
|
NISTAR |
|
NS |
- |