| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Towers, Zachary D (20)
|
NIUN-V |
52.45 |
53.19 |
0.74 |
| 2 |
Otto, Luke T (22)
|
NIVELO |
52.05 |
54.30 |
2.25 |
| 3 |
Bocci, David J (15)
|
LELESD |
57.78 |
56.74 |
-1.04 |
| 4 |
Acosta, Isaiah J (17)
|
NISTAR |
55.69 |
57.00 |
1.31 |
| 5 |
Matteson, Carter J (16)
|
NISTAR |
1:01.10 |
59.47 |
-1.63 |
| 6 |
Staples, Graeme A (15)
|
NISTAR |
58.35 |
1:01.37 |
3.02 |
| 7 |
Burkett, Robert O (15)
|
NISTAR |
1:03.27 |
1:03.49 |
0.22 |
| 8 |
Eisenmann, Zachary M (15)
|
NISTAR |
1:07.39 |
1:04.19 |
-3.20 |
| 9 |
Strittmatter, Blaise A (15)
|
NISTAR |
1:02.59 |
1:06.01 |
3.42 |
| 10 |
Nienhaus, Jack (16)
|
NISHAQ |
|
1:10.24 |
- |
| 11 |
Switalski, Nicholas M (16)
|
NITTSC |
1:15.65 |
1:20.24 |
4.59 |
|
Pierce, Talyn E (16)
|
NIVICT |
1:00.44 |
NS |
- |