| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reilly, Bridget F (9)
|
NISTAR |
34.58 |
34.39 |
-0.19 |
| 2 |
Golden, Lauren B (9)
|
NIBAAC |
34.89 |
38.11 |
3.22 |
| 3 |
Alessi, Luciana M (9)
|
NIBAAC |
37.86 |
38.27 |
0.41 |
| 4 |
Burnham, Madeline (9)
|
NIVELO |
43.41 |
39.28 |
-4.13 |
| 5 |
Puzanovova, Veronika A (9)
|
NIBAAC |
38.06 |
40.06 |
2.00 |
| 6 |
Rasmusson, Henrietta E (9)
|
NICLSC |
41.11 |
40.56 |
-0.55 |
| 7 |
Hutton, Ryan (9)
|
NIVELO |
45.35 |
41.66 |
-3.69 |
| 8 |
Ryan, Isabella A (9)
|
NIVELO |
39.11 |
42.19 |
3.08 |
| 9 |
Tunkey, Mila R (9)
|
NIEAST |
41.16 |
43.69 |
2.53 |
| 10 |
Washington, Ashanti M (9)
|
NISTAR |
42.22 |
44.39 |
2.17 |
| 11 |
Vuich, Layla J (9)
|
NISTAR |
1:07.19 |
1:04.10 |
-3.09 |
| 12 |
Persichini, Olivia L (9)
|
NISTAR |
1:05.23 |
1:14.48 |
9.25 |