| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gachette, Marisa B (10)
|
NIVELO |
34.02 |
32.74 |
-1.28 |
| 2 |
Anderson, Madden (10)
|
NIVELO |
36.46 |
34.10 |
-2.36 |
| 3 |
Kasubowski, Cassie A (10)
|
NIUN-E |
|
34.63 |
- |
| 4 |
Reif, Rydell (10)
|
NISHAQ |
35.53 |
34.79 |
-0.74 |
| 5 |
Barillari, Emma (10)
|
NIVELO |
36.14 |
34.82 |
-1.32 |
| 6 |
Strittmatter, Amelia C (10)
|
NISTAR |
36.83 |
34.90 |
-1.93 |
| 7 |
Philbrick, Mylie E (10)
|
NIVELO |
34.49 |
35.55 |
1.06 |
| 8 |
Zinno, Gianna M (10)
|
NICLSC |
37.80 |
36.55 |
-1.25 |
| 9 |
Weisenburg, Brylee M (10)
|
NISTAR |
38.28 |
36.99 |
-1.29 |
| 10 |
Reukauf, Anna R (10)
|
NISTAR |
38.54 |
37.05 |
-1.49 |
| 11 |
Odojewski, Molly L (10)
|
NISHAQ |
43.62 |
39.77 |
-3.85 |
| 12 |
Umansky, Isabella C (10)
|
NIBAAC |
39.44 |
39.96 |
0.52 |
| 13 |
Urbanek, Addison E (10)
|
NICLSC |
39.06 |
40.72 |
1.66 |
| 14 |
Crozier, Sydney (10)
|
NIVELO |
43.21 |
42.32 |
-0.89 |
| 15 |
Balcom, Lucy W (10)
|
NIBAAC |
|
44.40 |
- |
| 16 |
Barrera-Hagner, Ananya S (10)
|
NIEAST |
44.27 |
45.00 |
0.73 |
| 17 |
Gawron, Samantha G (10)
|
NICLSC |
50.39 |
45.49 |
-4.90 |
| 18 |
Tothero, Madeleine S (10)
|
NISTAR |
46.57 |
45.78 |
-0.79 |
| 19 |
Sirianni, Annabella A (10)
|
NISTAR |
|
52.79 |
- |
|
King, Skylar L (10)
|
NIEAST |
35.45 |
NS |
- |
|
Arcadi, Kayla A (10)
|
NIVELO |
|
NS |
- |
|
Wamsley, Sarah E (10)
|
NIVELO |
33.90 |
NS |
- |