| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Killion, Natalie A (12)
|
NICLSC |
26.38 |
25.61 |
-0.77 |
| 2 |
Leighton, Evelyn S (12)
|
NISTAR |
25.91 |
26.67 |
0.76 |
| 3 |
Amer, Aya F (12)
|
NIVELO |
30.79 |
29.67 |
-1.12 |
| 4 |
Vaccaro, Vanessa A (12)
|
NIBAAC |
28.38 |
29.78 |
1.40 |
| 5 |
Lin, Isabella E (12)
|
NIVELO |
30.76 |
30.20 |
-0.56 |
| 6 |
Pike, Olivia R (12)
|
NISTAR |
29.68 |
30.29 |
0.61 |
| 7 |
Domster, Madeline N (12)
|
NISTAR |
32.40 |
30.65 |
-1.75 |
| 8 |
Pirigyi, Sophia J (12)
|
NISTAR |
30.93 |
30.70 |
-0.23 |
| 9 |
Brown, Haley C (12)
|
NIVELO |
|
32.47 |
- |
| 10 |
Colder, Amelia W (12)
|
NISTAR |
34.27 |
32.82 |
-1.45 |
| 11 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
32.54 |
32.93 |
0.39 |
| 12 |
Hull, Emily G (12)
|
NISTAR |
32.50 |
33.82 |
1.32 |
| 13 |
Fosberg, Ella G (12)
|
NISTAR |
33.85 |
33.88 |
0.03 |
| 14 |
Nadarajan, Gouripriya (12)
|
NIVELO |
|
34.55 |
- |
| 15 |
Eisenmann, Abigail E (12)
|
NISTAR |
37.08 |
35.01 |
-2.07 |
| 16 |
Denham, Myanna M (12)
|
NISTAR |
36.15 |
36.48 |
0.33 |
| 17 |
Morelli, Emily G (12)
|
NICLSC |
39.05 |
37.42 |
-1.63 |
| 18 |
Tothero, Katherine M (12)
|
NISTAR |
37.94 |
37.53 |
-0.41 |
| 19 |
Delille, Beatrice F (12)
|
NIVELO |
44.24 |
38.53 |
-5.71 |
| 20 |
Tripi, Haylee E (12)
|
NIVELO |
39.33 |
39.16 |
-0.17 |
|
Tesluk, Elizabeth D (12)
|
NISTAR |
28.68 |
NS |
- |