| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Staples, Graeme A (16)
|
NISTAR |
2:20.91 |
2:26.48 |
5.57 |
| 2 |
Acosta, Isaiah J (18)
|
NISTAR |
2:31.82 |
2:29.54 |
-2.28 |
| 3 |
Blake, Joseph C (16)
|
NISTAR |
2:19.95 |
2:29.79 |
9.84 |
| 4 |
Walters, Zachary J (16)
|
NITWST |
2:30.12 |
2:32.00 |
1.88 |
| 5 |
Wangelin, Charlie L (15)
|
NITWST |
3:02.05 |
2:36.13 |
-25.92 |
| 6 |
Eisenmann, Zachary M (15)
|
NISTAR |
2:39.39 |
2:41.23 |
1.84 |
| 7 |
Matteson, Carter J (17)
|
NISTAR |
2:34.44 |
2:43.80 |
9.36 |
| 8 |
Sippel, Liam D (15)
|
NITWST |
2:57.76 |
2:46.30 |
-11.46 |
| 9 |
Choroser, Philip E (17)
|
NITWST |
3:21.73 |
2:47.52 |
-34.21 |
| 10 |
McCrone, Patrick (15)
|
NITWST |
|
2:47.76 |
- |
| 11 |
Strittmatter, Blaise A (15)
|
NISTAR |
2:53.26 |
2:48.70 |
-4.56 |
|
Morgan, Phillip A (16)
|
NIMERC |
2:51.73 |
NS |
- |