| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reif, Rydell (10)
|
NISHAQ |
|
43.54 |
- |
| 2 |
Anderson, Madden (10)
|
NIVELO |
45.36 |
43.55 |
-1.81 |
| 3 |
Strittmatter, Amelia C (10)
|
NISTAR |
40.55 |
43.78 |
3.23 |
| 4 |
Wamsley, Sarah E (10)
|
NIVELO |
46.66 |
45.52 |
-1.14 |
| 5 |
Umansky, Isabella C (10)
|
NIBAAC |
50.23 |
47.55 |
-2.68 |
| 6 |
Marchese, Cara E (10)
|
NIBAAC |
48.47 |
48.21 |
-0.26 |
| 7 |
Reukauf, Anna R (10)
|
NISTAR |
52.83 |
54.48 |
1.65 |
| 8 |
Arcadi, Kayla A (10)
|
NIVELO |
1:02.61 |
57.83 |
-4.78 |
| 9 |
Crozier, Sydney (10)
|
NIVELO |
58.97 |
58.34 |
-0.63 |
| 10 |
Barrera-Hagner, Ananya S (10)
|
NIEAST |
1:02.23 |
1:01.87 |
-0.36 |
|
Balcom, Lucy W (10)
|
NIBAAC |
53.19 |
NS |
- |
|
Morey, Celia R (10)
|
NIBAAC |
1:02.95 |
NS |
- |
|
Tothero, Madeleine S (10)
|
NISTAR |
56.25 |
NS |
- |