| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Killion, Natalie A (12)
|
NICLSC |
28.95 |
27.89 |
-1.06 |
| 2 |
Leighton, Evelyn S (12)
|
NISTAR |
32.23 |
30.92 |
-1.31 |
| 3 |
Amer, Aya F (12)
|
NIVELO |
32.44 |
33.24 |
0.80 |
| 4 |
Hull, Emily G (12)
|
NISTAR |
31.74 |
34.24 |
2.50 |
| 5 |
Lin, Isabella E (12)
|
NIVELO |
35.06 |
34.93 |
-0.13 |
| 6 |
Pike, Olivia R (12)
|
NISTAR |
39.24 |
35.05 |
-4.19 |
| 7 |
Domster, Madeline N (12)
|
NISTAR |
42.13 |
35.65 |
-6.48 |
| 8 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
34.19 |
36.67 |
2.48 |
| 9 |
Pirigyi, Sophia J (12)
|
NISTAR |
36.95 |
37.16 |
0.21 |
| 10 |
Colder, Amelia W (12)
|
NISTAR |
41.49 |
39.71 |
-1.78 |
| 11 |
Fosberg, Ella G (12)
|
NISTAR |
40.86 |
40.19 |
-0.67 |
| 12 |
Denham, Myanna M (12)
|
NISTAR |
|
42.39 |
- |
| 13 |
Brown, Haley C (12)
|
NIVELO |
|
42.61 |
- |
| 14 |
Tothero, Katherine M (12)
|
NISTAR |
48.65 |
42.84 |
-5.81 |
| 15 |
Nadarajan, Gouripriya (12)
|
NIVELO |
|
42.85 |
- |
| 16 |
Eisenmann, Abigail E (12)
|
NISTAR |
51.44 |
50.47 |
-0.97 |
| 17 |
Morelli, Emily G (12)
|
NICLSC |
54.69 |
50.89 |
-3.80 |
| 18 |
Tripi, Haylee E (12)
|
NIVELO |
|
53.95 |
- |
| 19 |
Delille, Beatrice F (12)
|
NIVELO |
|
1:02.06 |
- |
|
Tesluk, Elizabeth D (12)
|
NISTAR |
30.08 |
NS |
- |
|
Vaccaro, Vanessa A (12)
|
NIBAAC |
34.62 |
NS |
- |