| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hodge, Ashley S (12)
|
NIUN-D |
2:41.43 |
2:39.68 |
-1.75 |
| 2 |
Wu, Helen (12)
|
NIUN-D |
2:46.71 |
2:41.47 |
-5.24 |
| 3 |
Bauer, Lauren A (12)
|
NICLSC |
3:00.74 |
2:44.31 |
-16.43 |
| 4 |
Leighton, Evelyn S (11)
|
NISTAR |
3:39.34 |
2:53.56 |
-45.78 |
| 5 |
Kramer, Kyla A (12)
|
AMMIST |
3:01.77 |
2:54.78 |
-6.99 |
| 6 |
Moore, Paige E (12)
|
NIUN-D |
3:04.54 |
2:56.45 |
-8.09 |
| 7 |
Costich, Charlotte G (12)
|
NICLSC |
3:08.01 |
2:57.04 |
-10.97 |
| 8 |
Cordle, Gracie A (12)
|
NIFALC |
3:00.58 |
3:00.66 |
0.08 |
| 9 |
Keller, Mira (11)
|
NIVELO |
|
3:06.81 |
- |
| 10 |
Clouser, Kristiana I (12)
|
NIBCSR |
3:09.18 |
3:08.77 |
-0.41 |
| 11 |
Amer, Aya F (11)
|
NIVELO |
|
3:10.78 |
- |
| 12 |
Hughes, Samantha G (11)
|
NICLSC |
3:34.94 |
3:12.71 |
-22.23 |
| 13 |
Chaplin, Allison V (12)
|
NITTSC |
3:15.75 |
3:13.07 |
-2.68 |
| 14 |
Domster, Madeline N (12)
|
NISTAR |
|
3:14.25 |
- |
| 15 |
Pirigyi, Sophia J (11)
|
NISTAR |
3:15.65 |
3:14.67 |
-0.98 |
| 16 |
Berdysiak, Elizabeth C (11)
|
NICLSC |
|
3:15.56 |
- |
| 17 |
Pigeon, Aimee J (11)
|
NISTAR |
|
3:19.77 |
- |
| 18 |
Pasieka, Vivian C (11)
|
NICLSC |
3:33.76 |
3:22.20 |
-11.56 |
| 19 |
Wands, Amelia G (11)
|
NIVELO |
|
3:25.96 |
- |
| 20 |
Pike, Olivia R (11)
|
NISTAR |
|
3:31.79 |
- |
| 21 |
Kozacki, Natalie A (12)
|
NIBAAC |
3:28.14 |
3:37.40 |
9.26 |
| 22 |
Klose, Shayna (11)
|
NIBAAC |
|
3:40.87 |
- |
| 23 |
Polek, Sarah G (12)
|
NITTSC |
3:44.31 |
3:43.37 |
-0.94 |
| 24 |
Reimann, Meghan R (11)
|
NISTAR |
|
3:50.04 |
- |