| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wu, Helen (12)
|
NIUN-D |
3:09.94 |
3:02.76 |
-7.18 |
| 2 |
Leighton, Evelyn S (11)
|
NISTAR |
3:16.08 |
3:12.48 |
-3.60 |
| 3 |
Noecker, Elle A (11)
|
NITWST |
3:29.18 |
3:12.64 |
-16.54 |
| 4 |
Moore, Paige E (12)
|
NIUN-D |
3:19.23 |
3:15.76 |
-3.47 |
| 5 |
Tesluk, Elizabeth D (11)
|
NISTAR |
3:16.72 |
3:26.45 |
9.73 |
| 6 |
Fragale, Madison J (12)
|
NICLSC |
3:28.03 |
3:28.37 |
0.34 |
| 7 |
Clouser, Kristiana I (12)
|
NIBCSR |
3:47.33 |
3:28.72 |
-18.61 |
| 8 |
Kowell-Ure, Phoenix W (12)
|
NIEAST |
3:50.12 |
3:35.32 |
-14.80 |
| 8 |
Pigeon, Aimee J (11)
|
NISTAR |
3:45.67 |
3:35.32 |
-10.35 |
| 10 |
Mason, Henley N (12)
|
NICLSC |
|
3:39.87 |
- |
| 11 |
Amer, Aya F (12)
|
NIVELO |
3:42.68 |
3:49.63 |
6.95 |
| 12 |
Wands, Amelia G (11)
|
NIVELO |
3:51.61 |
3:53.67 |
2.06 |
| 13 |
Chung, Emily (11)
|
NIBAAC |
|
3:57.73 |
- |
| 14 |
Hauser-Howells, Lillian K (12)
|
NITTSC |
4:00.96 |
4:01.85 |
0.89 |